This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.The chapter Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues.- Principles of Ice-Free Cryopreservation by Vitrification.- The Principles of Freeze-Drying and Application of Analytical Technologies.- Mathematical Modeling and Optimization of Cryopreservation in Single Cells.- Mathematical Modeling of Protectant Transport in Tissues.- Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation.- Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics.- High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics.- Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization.- DSC Analysis of Thermophysical Properties for Biomaterials and Formulations.- Osmometric Measurements of Cryoprotective Agent Permeation into Tissues.- Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes.- Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research.- Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements.- Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm.- Cryopreservation of Avian Semen.- Cryopreservation of Mouse Sperm for Genome Banking.- Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages.- Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent.- Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage.- Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen.- Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research.- Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices.- Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts.- Vitrificationof Equine In Vivo-Derived Embryos, after Blastocoel Aspiration.- Frozen Blood Reserves.- Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes.- Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells.- Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem Cells.- Protocol for Cryopreservation of Endothelial Monolayers.- Vitrification of Heart Valve Tissues.- Cryopreservation of Algae.- Cryopreservation of Fern Spores and Pollen.- Cryopreservation of Plant Cell Lines Using Alginate Encapsulation.- Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3.- Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species.- Freeze-Drying of Proteins.- Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties.- Preservation of Mammalian Sperm by Freeze-Drying.- Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability.