Clinical Applications of Mass Spectrometry in Drug Analysis

This volume describes methods and protocols for a number of drugs and toxins in a stepwise manner. Chapters in the book cover a wide array of topics such as: quantitation of Flecainide, Mexiletine, Propafenone, and Amiodarone in Serum or Plasma; quantitation of total Buprenorphine and Norbuprenorphine in Meconium; quantitation or Carisoprodol and Meprobamate in Urine; and quantitation of Tricyclic Antidepressants in Serum. Each chapter contains a brief introduction to the topic, clinical utility of the analyte(s), and useful notes to help laboratorians easily reproduce the protocols discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Clinical Applications of Mass Spectrometry in Drug Analysis: Methods and Protocols, is a great resource for laboratorians who are already using mass spectrometry or thinking of introducing this technology to their laboratories.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice form the experts

Inhalt

Mass Spectrometry in Clinical Laboratory: Applications in Therapeutic Drug Monitoring and Toxicology.- Quantitation of Flecainide, Mexiletine, Propafenone, and Amiodarone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Quantitation of the Oral Anticoagulants Dabigatran, Rivaroxaban, Apixaban, and Warfarin in Plasma Using Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry (UPLC-MS/MS).- Simultaneous Quantitation of Lamotrigine, Levetiracetam, 10-Hydroxycarbazepine, Topiramate, and Zonisamide in Serum Using HPLC-MS/MS .- Quantification of the Triazole Antifungal Compounds Voriconazole and Posaconazole in Human Serum or Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS).- Quantitation of Haloperidol, Fluphenazine, Perphenazine, and Thiothixene in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Quantitation of Total Buprenorphine and Norbuprenorphine in Meconium by LC-MS/MS.- Quantitation of Buprenorphine, Norbuprenorphine, Buprenorphine Glucuronide, Norbuprenorphine Glucuronide, and Naloxone in Urine by LC-MS/MS.- A Simple Liquid Chromatography Tandem Mass Spectrometry Method for Quantification of Plasma Busulfan.- High-Throughput Quantitation of Busulfan in Plasma Using Ultra-Fast Solid Phase Extraction Tandem Mass Spectrometry (SPE-MS/MS).- Quantification of 11-Carboxy-Delta-9-Tetrahydrocannbinol (THC-COOH) in Meconium Using Gas Chromatography Mass Spectrometry (GC/MS).- Quantitation of Carisoprodol and Meprobamate in Urine and Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Cetirizine Quantification by High Performance Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS).- Quantification of Docetaxel in Serum UsingTurbulent Flow Liquid Chromatography Electrospray Tandem Mass Spectrometry (TFC-HPLC-ESI-MS/MS).- Comprehensive Urine Drug Screen by Gas-Chromatography Mass Spectrometry (GC/MS).- Broad Spectrum Drug Screening Using Liquid Chromatography-Hybrid Triple Quadrupole Linear Ion Trap Mass Spectrometry.- High Resolution Mass Spectrometry for Untargeted Drug Screening.- Quantitation of Ethyl Glucuronide and Ethyl Sulfate in Urine Using Liquid Chromatography-Tandem Mass Spectrometry(LC-MS/MS).- Quantification of Hydroxychloroquine in Blood Using Turbulent Flow Liquid Chromatography-Tandem mass Soectrometry (TFLC-MS/MS).- Quantification of Iohexol in Serum by High Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).- Quantitation of Teriflunomide in Human Serum/Plasma Across a 40,000-Fold Concentration Range by LC/MS/MS.- Determination of Menthol in Plasma and Urine by Gas Chromatography-Mass Spectrometry (GC/MS).- Development of an Assay for Methotrexate and its Metabolites 7-Hydroxy Methotrexate and DAMPA in Serum by LC-MS/MS.- Quantitative, Multidrug Pain Medication Testing by Liquid Chromatography Tandem Mass Spectrometry (LC-MSMS).- Quantification of Free Phenytoin by Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS).- Detection of Stimulants and Narcotics by Liquid Chromatography Tandem Mass Spectrometry and Gas Chromatography Mass Spectrometry for Sports Doping Control.- Quantification of Tricyclic Antidepressants in Serum Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS).