Circadian Regulation

This volume details methods on several aspects of circadian research. Chapters guide readers through the latest techniques and a wide variety of daily rhythmic processes, model organisms, circadian rhythms in the SCN and in peripheral organs, and describing in vitro systems and in silico methods. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Circadian Regulation: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Chapters 3, 4, 8, and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Sleep under Preindustrial Conditions: What We Can Learn from It.- The structure-based molecular-docking screen against core clock proteins to identify small molecules to modulate the circadian clock.- Analysis of complex circadian time series data using wavelets.- Mathematical Modeling in Circadian Rhythmicity.- Bioinformatics and Systems Biology of Circadian Rhythms: BIO CYCLE and CircadiOmics.- Cell-based Phenotypic Screens to Discover Circadian Clock Modulating Compounds.- Methods for Assessing Circadian Rhythms and Cell Cycle in Intestinal Enteroids.- Using ALLIGATORs to capture circadian bioluminescence.- Studying circadian clock entrainment by hormonal signals.- In vitro assays for measuring intercellular coupling among peripheral circadian oscillators.- Circadian control of transcriptional and metabolic rhythms in primary hepatocytes .- Electrophysiology of the Suprachiasmatic Nucleus: Single-Unit Recording.- AnatomicalMethods to Study the Suprachiasmatic Nucleus.- Circadian analysis of rodent locomotor activity in home cages.- Recording of diurnal gene expression in peripheral organs of mice using the RT-Biolumicorder.- Isolation and sorting of epidermal interfollicular stem cells for the study of circadian rhythms.- Detecting circadian rhythms in human red blood cells by dielectrophoresis.- Measuring circadian neutrophil infiltration in tissues by paired whole-mount tissue-clearing and flow cytometry.- In vivo imaging of circadian NET formation during lung injury by 4-dimensional intravital microscopy.- Real-time measurement of energy metabolism over circadian time using indirect calorimetry-enabled metabolic cages.- Untargeted and Targeted Circadian Metabolomics Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) and Flow Injection-Electrospray Ionization-Tandem Mass Spectrometry (FIA-ESI-MS/MS).- Time Restricted Feeding and Caloric Restriction - two feeding regimen at the crossroad of metabolic and circadian regulation. -Chromatin Immunoprecipitation and Circadian Rhythms.- Fluorescent reporters for studying circadian rhythms in Drosophila melanogaster.- Visualization of mutant aggregates from clock neurons by agarose gel electrophoresis (AGERA) in Drosophila melanogaster.- Methods for delivery of dsRNAi against canonical clock genes and immunocytodetection of clock proteins in crustacea.- In vivo bioluminescence analyses of circadian rhythms in Arabidopsis thaliana using a microplate luminometer.