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Chloroplast Research in Arabidopsis

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Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied... Weiterlesen
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Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable. The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid. In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

Brings together the most important and current techniques in chloroplast research through the unifying lens of the Arabidopsis model system

Features subjects like protein aspects, omics studies, and photosynthesis and biochemical analysis

Includes expert tips and implementation advice to guarantee ideal experimental results


Part I: Multiprotein Complexes and Protein-Protein Interactions

1. One- and Two-Dimensional Blue Native (BN) PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes

Shingo Kikuchi, Jocelyn Bédard, and Masato Nakai

2. Analysis of Thylakoid Protein Complexes by Two Dimensional Electrophoretic Systems

Sari Sirpiö, Marjaana Suorsa, and Eva-Mari Aro

3. Preparation of Multiprotein Complexes from Arabidopsis Chloroplasts Using Tandem Affinity Purification

Charles Andrès, Birgit Agne, and Felix Kessler

4. Studying Interactions between Chloroplast Proteins in Intact Plant Cells Using Bimolecular Fluorescence Complementation (BiFC) and Förster Resonance Energy Transfer (FRET)

Jodi Maple and Simon G. Møller

5. Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods

Joanna Tripp and Enrico Schleiff

Part II: Omics and Large-Scale Analyses

6. Proteome Databases and Other Online Resources for Chloroplast Research in Arabidopsis

Diogo Ribeiro Demartini, Célia Regina Carlini, and Jay J. Thelen

7. Use of Transcriptomics to Analyse Chloroplast Processes in Arabidopsis

Tatjana Kleine and Dario Leister

8. Use of Non-Aqueous Fractionation and Metabolomics to Study Chloroplast Function in Arabidopsis

Peter Geigenberger, Axel Tiessen, and Jörg Meurer

9. Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis

Yan Lu, Linda J. Savage, and Robert L. Last

Part III: Proteomics and Suborganellar Fractionation

10. Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies

Daniel Salvi, Lucas Moyet, Daphné Seigneurin-Berny, Myriam Ferro, Jacques Joyard, and Norbert Rolland

11. Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic Analysis

Michael Hall, Yogesh Mishra, and Wolfgang P. Schröder

12. Preparation of Plastoglobules from Arabidopsis Plastids for Proteomic Analysis and Other Studies

Celine Besagni, Lucia Eugeni Piller, and Claire Bréhélin

13. Preparation and Proteomic Analysis of Chloroplast Ribosomes

Kenichi Yamaguchi

14. The Workflow for Quantitative Proteome Analysis of Chloroplast Development and Differentiation, Chloroplast Mutants, and Protein Interactions by Spectral Counting

Giulia Friso, Paul Dominic B. Olinares, and Klaas J. van Wijk

15. Use of Phosphoproteomics to Study Post-Translational Protein Modifications in Arabidopsis Chloroplasts

Anne Endler and Sacha Baginsky

Part IV: Photosynthesis and Biochemical Analysis

16. Use of a Pulse-Amplitude Modulated (PAM) Chlorophyll Fluorometer to Study the Efficiency of Photosynthesis in Arabidopsis Plants

Matthew D. Brooks and Krishna K. Niyogi

17. Gas Exchange Measurements for the Determination of Photosynthetic Efficiency in Arabidopsis Leaves

Giles Johnson and Erik Murchie

18. Measurement of the pH and Electric Field Developed Across Arabidopsis Thylakoids in the Light

Steven M. Theg and Curtis Tom

19. Measurement of Chloroplast ATP Synthesis Activity in Arabidopsis

Aleel K. Grennan and Donald R. Ort

20. Methods for Analysis of Photosynthetic Pigments and Steady-State Levels of Intermediates of Tetrapyrrole Biosynthesis

Olaf Czarnecki, Enrico Peter, and Bernhard Grimm

21. Analysis of Starch Metabolism in Chloroplasts

Carmen Hostettler, Katharina Kölling, Diana Santelia, Sebastian Streb, Oliver Kötting, and Samuel C. Zeeman

22. The Analysis of Lipid Content and Quality in Arabidopsis Plastids

Anna Maria Zbierzak, Peter Dörmann, and Georg Hölzl


Titel: Chloroplast Research in Arabidopsis
Untertitel: Methods and Protocols, Volume II
EAN: 9781617792366
ISBN: 1617792365
Format: Fester Einband
Herausgeber: Humana Press
Anzahl Seiten: 444
Gewicht: 1026g
Größe: H260mm x B183mm x T27mm
Jahr: 2011
Untertitel: Englisch
Auflage: 2011

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