This volume illustrates techniques on solation, purification of axenic Chlorella spp.. Chapters guide readers on natural water, morphological identification using microscopic morphology, molecular characterization and identification of Chlorella Species, 18S rRNA gene sequencing, phylogenetic analysis, whole genome analysis of Chlorella using Illumina Hiseq, genome characterization using GeSeq, and metabolic pathway analysis using KEGG.

Authoritative and cutting-edge, Chlorella Genomics, Cultivation, and Bioenterpise: Green Economy through Algal Innovations aims to be a comprehensive resource for both academic and industrial applications.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Zusammenfassung
This volume illustrates techniques on solation, purification of axenic Chlorella spp.. font-family: 'Times New Roman',serif;">phylogenetic analysis,

Inhalt

Collection and Initial Analysis of Water Samples Potentially Containing Chlorella spp. from a Freshwater Environment.- Isolation of Chlorella spp. from natural water using serial dilution and micropipette techniques.- Purification of axenic Chlorella spp. cultures using streak plate and antibiotic treatment.- Wet Mount Preparation and Microscopic Examination of Chlorella vulgaris, Chlorella sorokiniana, Chlorella pyrenoidosa, Chlorella minutissima Chlorella protothecoides .- Examination of Live and dead Chlorella cells using fluorescence staining.- Identification of Chlorella and Bacterial Signalling Molecules.- Isolation and identification of bacterial symbionts from Chlorella phycosphere symbionts.- Isolation and identification of actinobacteria associated in the phycosphere of Chlorella.- Separation of Amplified Gene Using Agarose Gel Electrophoresis.- PCR Amplification of 18S rRNA gene in Chlorella sp..- Separation of Amplified Gene Using Agarose Gel Electrophoresis.- 18S rRNA Gene Sequencing and BLAST Analysis Using NCBI.- Phylogenetic analysis of 18S rRNA gene in Chlorella using Neighbor-Joining (NJ) tree.- Whole genome sequencing of Chlorella Using Illumina HiSeq.- Chlorella genome assembly with SPAdes and annotation using GenSAS.- Genome characterization of Chlorella using GeSeq.- Metabolic pathway analysis of the Chlorella genome using eggNOG-mapper and KEGG.- CRISPR-Cas9 Guide RNA Designing for Genome Editing in Chlorella sp. using CHOP-CHOP.- Comparative genome analysis of Chlorella species.- Preparation of Starter Culture of Chlorella .- Coculture of Chlorella and Bacteria for Enhanced Biomass Production.- Scaling up of Chlorella spp from lab to pilot scale.- Large Scale Cultivation of Chlorella in Open Pond Systems.- Harvesting Techniques of Chlorella Biomass using Filtration and Sedimentation.- PostHarvest Processing of Chlorella Biomass - Drying and Powdering.- Harvesting techniques of Chlorella biomass using filtration and sedimentation.- PostHarvest Processing of Chlorella Biomass by Drying and Powdering.- Fish Feed Formulation with Chlorella Wet Paste Form (Live/Concentrated Feed).- Production of Chlorella Dry Powder as a Value-Added Supplemental Feed.- Chlorella enriched Feed Pellet Preparation.- Nutritional Analysis of Chlorella Aquafeed.- Chlorella Aquafeed Packaging and Labelling.- Experimental Validation of Chlorella -Enriched Feed in Tilapia Fish.- Preparation of Dietary Supplements and Functional Foods using Chlorella sp. .- Preparation of Polyunsaturated Fatty Acids (PUFAs): Omega-3 and Omega-6 Fatty Acids from Chlorella sp..- Preparation and quality assessment of Chlorella Green Smoothie .- Preparation, nutritional and sensory analysis of Chlorella based Cookies.