Methods for enantiomer separation are very important for controlling synthesis, for checking racemization, for enantiomeric purity control, and for pharmacokinetic studies. In Chiral Separations: Methods and Protocols, prominent experts from around the world detail the chromatographic and electroseparation techniques they have developed for chiral separations on an analytical scale. Described in step-by-step detail to ensure successful experimental results, the procedures are presented as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques, but also including thin layer chromotogrphic, gas chromatographic, and supercritical fluid chromatographic as well as recent electrochromotographic techniques. Each fully tested protocol includes an introduction to the principle underlying the method, equipment and reagent lists, and helpful notes on troubleshooting and avoiding known pitfalls.
Up-to-date and highly practical, Chiral Separations: Methods and Protocols will help both novice and advanced users not only choose optimal methods for their separation problems, but also readily obtain successful results.

Includes supplementary material: sn.pub/extras

Zusammenfassung
Details chromatographic and electroseparation techniques developed for chiral separations on an analytical scale. This text presents procedures as either general methods or as specific applications to substance classes and special compounds, with emphasis on high performance liquid chromatography and capillary electrophoresis techniques.

Inhalt
Chiral Separation Principles.- Separation of Enantiomers by Thin-Layer Chromatography.- Cyclodextrin-Based Chiral Stationary Phases for Liquid Chromatography.- Enantiomeric Separations by HPLC Using Macrocyclic Glycopeptide-Based Chiral Stationary Phases.- Chiral Separation by HPLC Using Polysaccharide-Based Chiral Stationary Phases.- Applications of Polysaccharide-Based Chiral Stationary Phases for Resolution of Different Compound Classes.- Chiral Separation by HPLC With Pirkle-Type Chiral Stationary Phases.- Chiral Separation by HPLC Using the Ligand-Exchange Principle.- Chiral Separations by HPLC Using Molecularly Imprinted Polymers.- Indirect Enantioseparation by HPLC Using Chiral Benzofurazan-Bearing Reagents.- Separation of Racemic Trans-Stilbene Oxide by Sub-/Supercritical Fluid Chromatography.- Chiral Separations Using the Macrocyclic Antibiotics in Capillary Electrophoresis.- Enantioresolutions by Capillary Electrophoresis Using Glycopeptide Antibiotics.- Separation of Enantiomers by Capillary Electrophoresis Using Cyclodextrins.- Chiral Separations by Capillary Electrophoresis Using Proteins as Chiral Selectors.- Cellulases as Chiral Selectors in Capillary Electrophoresis.- Use of Chiral Crown Ethers in Capillary Electrophoresis.- Chiral Separations by Capillary Electrophoresis Using Cinchona Alkaloid Derivatives as Chiral Counter-Ions.- Chiral Separation by Capillary Electrophoresis Using Polysaccharides.- Chiral Micellar Electrokinetic Chromatography.- Chiral Separation by Capillary Electrophoresis in Nonaqueous Medium.- Chiral Ligand-Exchange Capillary Electrophoresis and Capillary Electrochromatography.- Enantioseparation in Capillary Chromatography and Capillary Electrochromatography Using Polysaccharide-Type Chiral Stationary Phases.- Chiral Separation by Capillary Electrochromatography Using Cyclodextrin Phases.- Chiral Separations by Capillary Electrochromatography Using Molecularly Imprinted Polymers.