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Characterization of Nanoparticles Intended for Drug Delivery

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In recent years, there have been many exciting breakthroughs in the application of nanotechnology to medicine. In Characterization... Weiterlesen
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Beschreibung

In recent years, there have been many exciting breakthroughs in the application of nanotechnology to medicine. In Characterization of Nanoparticles Intended for Drug Delivery, expert researchers explore the latest advances in the field, providing a set of basic methods for the characterization of nanomaterials for medical use. Chapters provide methods to characterize the physiochemical properties (size, aggregation, and surface chemistry) and in vitro immunological and biological characteristics of nanomaterials. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and informative, Characterization of Nanoparticles Intended for Drug Delivery is an essential survey of methods that are crucial to the preclinical characterization of nanomedicines.

Provides the reader with protocols that have been tested against clinically relevant nanoparticles

Describes some of the nuances of nanoparticle types and necessary controls

Gives a broad overview of applications of nanotechnology to medicine

Outlines the beneficial aspects of nanotechnology-based drug delivery



Zusammenfassung
In Characterization of Nanoparticles Intended for Drug Delivery, expert researchers explore the latest advances in the field, providing a set of basic methods for the characterization of nanomaterials for medical use.

Inhalt
Characterization of Nanoparticles Intended for Drug Delivery Table of Contents Part 1. Introduction and Discussion 1. Unique Benefits of Nanotechnology to Drug Delivery and Diagnostics Scott E. McNeil 2. Challenges for Nanoparticle Characterization Scott E. McNeil 3. Considerations when submitting nano-therapeutics to FDA/CDER for regulatory review Katherine Tyner & Nakissa Sadrieh Part 2. Assays to Determine Physical & Chemical Properties 4. Measuring the Hydrodynamic Size of Nanoparticles in Aqueous Media Using Batch-Mode Dynamic Light Scattering Vincent A. Hackley and Jeffrey D. Clogston 5. Characterization of Nanoparticles by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Uma Ramalinga, Jeffrey D. Clogston, Anil K. Patri, and John T. Simpson 6. Zeta Potential Measurement Jeffrey D. Clogston and Anil K. Patri 7. Size Measurement of Nanoparticles using Atomic Force Microscopy Jaroslaw Grobelny, Frank W. DelRio, Namboodirei Pradeep, Doo-In Kim, Vincent A. Hackley and Robert F. Cook 8. Biological Tissue and Cell Culture Specimen Preparation for TEM Nanoparticle Characterization Kunio Nagashima, Jiwen Zheng, David Parmiter and Anil K. Patri 9. SEM X-ray Microanalysis of Nanoparticles Present in Tissue or Cultured Cell Thin Sections Jiwen Zheng, Kunio Nagashima, David Parmiter, Jason de la Cruz and Anil K. Patri 10. Detecting and Measuring Free Gadolinium in Nanoparticles for MRI Imaging Jeffrey D. Clogston and Anil K. Patri 11. Lipid Component Quantitation by Thin Layer Chromatography Jeffrey D. Clogston & Anil K. Patri Part 3. Assays for Sterility 12. Detection and Quantitative Evaluation of Endotoxin Contamination in Nanoparticle Formulations by LAL-based Assays Barry W. Neun & Marina A. Dobrovolskaia 13. Analysis of microbial contamination in nanoparticle formulations Timothy M. Potter & Marina A. Dobrovolskaia Part 4. Assays for In Vitro Detection and Quantitation 14. Gold Nanoparticle Quantitation via Fluorescence in Solution and Cell Culture Parag Aggarwal & Marina A. Dobrovolskaia 15. Quantitation of Nanoparticles in Serum Matrix by Capillary Electrophoresis King C. Chan, Timothy D. Veenstra, and Haleem J. Issaq Part 5. In Vitro Assays for Toxicity 16. Evaluation of Cytotoxicity of Nanoparticulate Materials in Porcine Kidney Cells and Human Hepatocarcinoma Cells Timothy M. Potter and Stephan T. Stern 17. Monitoring Nanoparticle-Treated Hepatacarcinoma Cells for Apoptosis Timothy M. Potter and Stephan T. Stern 18. Detecting Reactive Oxygen Species in Primary Hepatocytes Treated with Nanoparticles Stephan T. Stern 19. Assay to Detect Lipid Peroxidation Upon Exposure to Nanoparticles Timothy M. Potter, Barry W. Neun, and Stephan T. Stern 20. Monitoring Glutathione Homeostasis in Nanoparticle-Treated Hepatocytes Timothy M. Potter, Barry W. Neun, and Stephan T. Stern 21. Autophagy Monitoring Assay: Qualitative Analysis of MAP LC3-I to II Conversion by Immunoblot Christopher B. McLeland, Jamie Rodriguez, and Stephan T. Stern 22. Monitoring Lysosomal Activity in Nanoparticle-Treated Cells Barry W. Neun and Stephan T. Stern Part 6. In Vitro Immunological Assays 23. Method for Analysis of Nanoparticle Hemolytic Properties in Vitro Barry W. Neun and Marina A. Dobrovolskaia 24. Method for In Vitro Analysis of Nanoparticle Thrombogenic Properties Barry W. Neun and Marina A. Dobrovolskaia 25. Qualitative Analysis of Total Complement Activation by Nanoparticles Barry W. Neun and Marina A. Dobrovolskaia 26. Method for Analysis of Nanoparticle Effects on Cellular Chemotaxis Timothy Potter, Sarah Skoczen & Marina A. Dobrovolskaia 27. In Vitro Analysis of Nanoparticle Uptake by Macrophages Using Chemiliuminescence Timothy Potter, Sarah Skoczen & Marina A. Dobrovolskaia

Produktinformationen

Titel: Characterization of Nanoparticles Intended for Drug Delivery
Editor:
EAN: 9781603271974
ISBN: 160327197X
Format: Fester Einband
Herausgeber: Springer-Verlag GmbH
Anzahl Seiten: 269
Gewicht: 691g
Größe: H263mm x B185mm x T23mm
Jahr: 2010
Auflage: 2011

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