This third edition summarizes the most important areas of Cell-Penetrating Peptides (CPP) research. Chapters are divided into three parts detailing the historical background of CPP, the classifications of the available CPPs, approaches for prediction of novel CPPs, methods for studies of naked CPPs, and a brief summary of functionality issues of CPPs, both in vitro and in vivo . Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Cell Penetrating Peptides: Methods and Protocols, Third Edition aims to be a useful practical guide to researches to help further their study in this field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible result Contains key notes and implementation advice from the experts?

Inhalt

Cell-penetrating peptides.- Shuttling homeoproteins and their biological significance.- Artificial evolutionary optimization process to improve the functionality of CPPs.- In silico design of chemically modified cell penetrating peptides.- Synthetic Molecular Evolution of Cell Penetrating Peptides.- A native CPP from rattlesnake with therapeutic and theranostic properties.- Synthesis of cell-penetrating peptide coated silica nanoparticles and their physicochemical and biological characterization.- Enhancing the therapeutic potential of extracellular vesicles using peptide technology.- Challenges and methods for the study of CPP translocation mechanisms.- Membrane molecular interactions and induced structures of CPPs.- A single GUV method for revealing the action of cell-penetrating peptides in biomembranes.- Methods for Small-Angle Scattering Measurements on Peptiplexes of DNA with Cell-Penetrating Peptides.- Imaging CPP delivery mechanisms of oligonucleotides.- Quantitative subcellular analysis of cyclic cell-penetrating peptide EJP18 in non-adherent cells.- PepFect14: signaling and transfection.- Utilisation of cell-penetrating peptides for in vivo delivery of bioactive cargo the effect of nanoparticle formulation.- Protein delivery by PTDs/CPPs.- Protein Delivery to Cytosol by Cell-Penetrating Peptide Bearing a Tandem Repeat Penetration-Accelerating Sequence.- Recombinant transcription factors (TFs) fused to ZEBRA minimal transduction domain (MD) for modulation of mRNA transcripts.- Protein mimicry and the design of bioactive cell penetrating peptides: The genesis of STOPSPERM bioportides.- Lipopeptide pepducins as therapeutic agents.- Delivery of Antibiotics by Cell-Penetrating Peptides to Kill Intracellular Pathogens.- CPP applications in immune modulation and disease therapy.- Cell-penetrating peptides as carriers for transepithelial drug delivery.- New tools for streamlined in vivo homing peptide identification.- Tumor targeting as new therapeutic approach.- Mitochondrial Targeting Probes, drug conjugates and gene therapeutics.- Novel brain penetrating single chain antibodies directed against 3RTau for the Treatment of Alzheimer's Disease and related Dementias.- Strategies for peptide-mediated cargo delivery to human smooth muscle cells.- Tips and Tools to Understand Direct Membrane Translocation of siRNA-Loaded WRAP-Based Nanoparticles.- Evaluating efficacy of peptide-delivered oligonucleotides using the severe Taiwanese SMA mouse model.- Development of CPP-based methods for delivery of miRNAs into the skin and airways - lessons from cell culture and mouse models.- Endpoint and kinetic approaches for assessing transfection efficacy in mammalian cell culture.- Tissue analysis of lung-targeted delivery of siRNA and plasmid DNA.- Improvement of transfection with PepFects byorganic and inorganic components.- DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent.- Use of PepFect14 and siRNA for targeted gene silencing in human embryonic stem cells.- CRISPR/Cas9 plasmid delivery through the CPP: PepFect14.- Applications of CPPs in genome editing of plants.- Live Cell Genomics: Cell-specific transcriptome capture and exon-specific RNA binding protein isolation in live tissues and cells.