Cell Imaging Techniques

A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Includes supplementary material: sn.pub/extras

Klappentext
Cell imaging methodologies have now become essential research tools for a variety of disciplines that traditionally had not relied on them. In Cell Imaging Techniques: Methods and Protocols, distinguished international researchers describe in detail their state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complementary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Timely and highly practical, Cell Imaging Techniques: Methods and Protocols provides researchers and clinicians with a richly useful guide to selecting and performing the best imaging method from a bewildering variety of microscopy-based techniques.

Inhalt
Molecular Beacons.- Second-Harmonic Imaging of Collagen.- Visualizing Calcium Signaling in Cells by Digitized Wide-Field and Confocal Fluorescent Microscopy.- Multifluorescence Labeling Techniques and Confocal Laser Scanning Microscopy on Lung Tissue.- Evaluation of Confocal Microscopy System Performance.- Quantitative Analysis of Atherosclerotic Lesion Composition in Mice.- Applications of Microscopy to Genetic Therapy of Cystic Fibrosis and Other Human Diseases.- Laser Scanning Cytometry.- Near-Clinical Applications of Laser Scanning Cytometry.- Laser Capture Microdissection.- Analysis of Asbestos-Induced Gene Expression Changes in Bronchiolar Epithelial Cells Using Laser Capture Microdissection and Quantitative Reverse Transcriptase-Polymerase Chain Reaction.- New Approaches to Fluorescence In Situ Hybridization.- Microarray Image Scanning.- Near-Field Scanning Optical Microscopy in Cell Biology and Cytogenetics.- Porosome.- Secretory Vesicle Swelling by Atomic Force Microscopy.- Imaging and Probing Cell Mechanical Properties With the Atomic Force Microscope.- Reflection Contrast Microscopy.- Three-Dimensional Analysis of Single Particles by Electron Microscopy.- Three-Dimensional Reconstruction of Single Particles in Electron Microscopy.- A New Microbiopsy System Enables Rapid Preparation of Tissue for High-Pressure Freezing.