Cell Imaging Techniques

Cell imaging methodologies have now become essential research tools for a variety of disciplines that traditionally had not relied on them. In Cell Imaging Techniques: Methods and Protocols, distinguished international researchers describe in detail their state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complementary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Timely and highly practical, Cell Imaging Techniques: Methods and Protocols provides researchers and clinicians with a richly useful guide to selecting and performing the best imaging method from a bewildering variety of microscopy-based techniques.

Klappentext

A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Zusammenfassung
A collection of methods for the microscopic imaging of cells and molecules, this work covers various complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. It discusses preparative methods for particles and cells by transmission electron microscopy.

Inhalt

Molecular Beacons: Fluorescent Probes for Detection of Endogenous mRNAs in Living Cells Diana P. Bratu Second-Harmonic Imaging of Collagen Guy Cox and Eleanor Kable Visualizing Calcium Signaling in Cells by Digitized Wide-Field and Confocal Fluorescent Microscopy Michael Wm. Roe, Jerome F. Fiekers, Louis H. Philipson, and Vytautas P. Bindokas Multifluorescence Labeling Techniques and Confocal Laser Scanning Microscopy on Lung Tissue Maria Stern, Douglas J. Taatjes, and Brooke T. Mossman Evaluation of Confocal Microscopy System Performance Robert M. Zucker Quantitative Analysis of Atherosclerotic Lesion Composition in Mice Marilyn P. Wadsworth, Burton E. Sobel, David J. Schneider, Wendy Tra, Hans van Hirtum, and Douglas J. Taatjes Applications of Microscopy to Genetic Therapy of Cystic Fibrosis and Other Human Diseases Thomas O. Moninger, Randy A. Nessler, and Kenneth C. Moore Laser Scanning Cytometry: Principles and Applications Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz Near-Clinical Applications of Laser Scanning Cytometry David A. Rew, Gerrit Woltmann, and Davinder Kaur Laser Capture Microdissection Virginia Espina, John Milia, Glendon Wu, Stacy Cowherd, and Lance A. Liotta Analysis of Asbestos-Induced Gene Expression Changes in Bronchiolar Epithelial Cells Using Laser Capture Microdissection and Quantitative Reverse Transcriptase-Polymerase Chain Reaction Christopher B. Manning, Brooke T. Mossman, and Douglas J. Taatjes New Approaches to Fluorescence In Situ Hybridization Sabita K. Murthy and Douglas J. Demetrick Microarray Image Scanning Latha Ramdas and Wei Zhang Near-Field Scanning Optical Microscopy in Cell Biology and Cytogenetics Michael Hausmann, Birgit Perner, Alexander Rapp, Leo Wollweber, Harry Scherthan, and Karl-Otto Greulich Porosome: The Fusion Pore Revealed by Multiple Imaging Modalities Bhanu P. Jena Secretory Vesicle Swelling by Atomic Force Microscopy Sang-Joon Cho and Bhanu P. Jena Imaging and Probing Cell Mechanical Properties With the Atomic Force Microscope Kevin D. Costa Reflection Contrast Microscopy: The Bridge Between Light and Electron Microscopy F. A. Prins, I. Cornelese-ten Velde, and E. de Heer Three-Dimensional Analysis of Single Particles by Electron Microscopy: Sample Preparation and Data Acquisition Teresa Ruiz and Michael Radermacher Three-Dimensional Reconstruction of Single Particles in Electron Microscopy: Image Processing Michael Radermacher and Teresa Ruiz A New Microbiopsy System Enables Rapid Preparation of Tissue for High-Pressure Freezing Dimitri Vanhecke, Peter Eggli, Werner Graber, and Daniel Studer Index