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Cell Cycle Synchronization

  • Fester Einband
  • 295 Seiten
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This Methods in Molecular Biology(TM) volume offers detailed protocols, with theoretical background, and step-by-step instructions on implementing synchronization, as well as current techniques for the enhanced study of regulatory mechanisms in cell cycle events.

To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the arrest-and-release approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.

Offers practical lab protocols for synchronizing mammalian, plant, yeast, fungal, and even bacterial cells

Serves a wide variety of scientists, from cell biologists to medical students to pharmacologists

Features key tips and expert implementation advice to ensure successful results

Includes supplementary material:


1. Overview of Cell Synchronization

Gaspar Banfalvi

2. Synchronization of Mammalian Cells and Nuclei by Centrifugal Elutriation

Gaspar Banfalvi

3. Cytofluorometric Purification of Diploid and Tetraploid Cancer Cells

Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Métivier, Mohamed Jèmaà, Santiago Rello-Varona, and Guido Kroemer

4. Large Scale Mitotic Cell Synchronization

Kalyan Dulla and Anna Santamaria

5. Synchronization of Mammalian Cell Cultures by Serum Deprivation

Thomas J. Langan and Richard C. Chou

6. Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Response: Analysis by Flow Cytometry

Zbigniew Darzynkiewicz, H. Dorota Halicka, Hong Zhao, and Monika Podhorecka

7. Chromosome Formation during Fertilization in Eggs of the Teleost, Oryzias latipes

Takashi Iwamatsu

8. Specific Cell Cycle Synchronization with Butyrate and Cell Cycle Analysis

Congjun Li

9. Analysis of Nuclear Uracil DNAGlycosylase (nUDG) Turnover during the Cell Cycle

Jennifer A. Fischer and Salvatore Caradonna

10. Synchronization of HeLa Cells

Hoi Tang Ma and Randy Y.C. Poon

11. Synchronization of Bacillus subtilis Cells by Spore Germination and Outgrowth

Gaspar Banfalvi

12. Synchronization of Yeast

Arkadi Manukyan, Lesley Abraham, Huzefa Dungrawala, and Brandt L. Schneider

13. Synchronization of Pathogenic Protozoans

Staffan Svärd and Karin Troell

14. Synchronization of In Vitro Maturation in Porcine Oocytes

Tamas Somfai and Yuji Hirao

15. Synchronization of Medicago sativa Cell Suspension Culture

Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, and Gábor V. Horváth

16. Cell Cycle Synchronization for the Purpose of Somatic Cell Nuclear Transfer (SCNT)

Yoel Shufaro and Benjamin E. Reubinoff

17. Ex Vivo Expansion of Hematopoietic Stem Cells to Improve Engraftment in Stem Cell Transplantation

Kap-Hyoun Ko, Robert Nordon, Tracey A. O'Brien, Geoff Symonds, and Alla Dolnikov

18. Flow Cytometry Developments and Perspectives in Clinical Studies: Examples in ICU Patients

Fabienne Venet, Caroline Guignant, and Guillaume Monneret

19. Molecular Network Dynamics of Cell Cycle Control: Transitions to Start and Finish

Attila Csikász-Nagy, Alida Palmisano, and Judit Zámborszky


Titel: Cell Cycle Synchronization
Untertitel: Methods and Protocols
EAN: 9781617791819
ISBN: 978-1-61779-181-9
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 295
Größe: H254mm x B254mm
Jahr: 2011
Untertitel: Englisch
Auflage: 2011.

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