A collection of new reviews and protocols from leading experts in cell cycle regulation, Cell Cycle Control: Mechanisms and Protocols, Second Edition presents a comprehensive guide to recent technical and theoretical advancements in the field. Beginning with the overviews of various cell cycle regulations, this title presents the most current protocols and state-of-the-art techniques used to generate latest findings in cell cycle regulation, such as protocols to analyze cell cycle events and molecules. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Cell Cycle Control: Mechanisms and Protocols, Second Edition will be a valuable resource for a wide audience, ranging from the experienced cell cycle researchers looking for new approaches to the junior graduate students giving their first steps in cell cycle research.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Cell Cycle-Regulated Transcription: Effectively Using a Genomics Toolbox.- Cell Cycle Regulation by Checkpoints.- Interplay Between the Cell Cycle and Double-Strand Break Response in Mammalian Cells.- Cell Cycle Regulation by Protein Degradation.- Linking Chromosome Duplication to Segregation Via Sister Chromatid Cohesion.- The Greatwall-PP2A Axis in Cell Cycle Control.- Cell Cycle Regulation by the Nutrient-Sensing Mammalian Target of Rapamycin (mTOR) Pathway.- The Senescence Arrest Program and the Cell Cycle.- Oncogenic Ras Pushes (and Pulls) Cell Cycle Progression Through ERK Activation.- Cell Cycle Regulation During Viral Infection.- The Roles of Cohesins in Mitosis, Meiosis, and Human Health and Disease.- Introductory Review of Computational Cell Cycle Modeling.- Cell Cycle Synchronization and Flow Cytometry Analysis of Mammalian Cells.- Analyzing Transcription Dynamics During the Budding Yeast Cell Cycle.- Analyzing Cell Cycle Checkpoints in Response to Ionizing Radiation in Mammalian Cells.- Analyzing DNA Replication Checkpoint in Budding Yeast.- Analyzing Cell Cycle-Dependent Degradation and Ubiquitination in Budding Yeast.- Imaging Analysis of Cell Cycle-Dependent Degradation of Cdt1 in Mammalian Cells.- PCNA-Dependent Ubiquitination of Cdt1 and p21 in Mammalian Cells.- Analyzing Cdc2/Cdk1 Activation During Stress Response in Schizosaccharomyces pombe.- Analyzing Ras-Associated Cell Proliferation Signaling.- Telomere Regulation During Cell Cycle in Fission Yeast.- Detecting Senescence: Methods and Approaches.- Analyzing RB and E2F During the G1/S Transition.- Analyzing G1/S Transcriptional Controls.- Analysis of Replication Timing Using Synchronized Budding Yeast Cultures.- Analysis of ssDNA Gaps and DSBs in Genetically Unstable Yeast Cultures.- Chromatin Fractionation Analysis of Licensing Factors in Mammalian Cells.- Imaging Analysis to Determine Chromatin Binding of Licensing Factors MCM2-7 in Mammalian Cells.- Chromatin Immunoprecipitation to Investigate Origin Association of Replication Factors in Mammalian Cells.- Live Cell Fluorescence Imaging for Phenotypic Analysis of Mitosis.- Analyzing Sister Chromatid Cohesion in Mammalian Cells.- Affinity Purification of Protein Complexes from Drosophila Embryos in Cell Cycle Studies.- Tracking Histone Variant Nucleosomes Across the Human Cell Cycle Using Biophysical, Biochemical and Cytological Analyses.