Research into gluten sensitivity has never been more popular nor more exciting. Thus a call for a new book, Celiac Disease: Methods and Protocols, devoted entirely to techniques and technology seemed a most appropriate undertaking. I am therefore grateful to Professor J. M. Walker for inviting me to complete this task for Humana Press. To do this would have been imp- sible without the contributions of friends and colleagues from around the world who have devoted so much interest to the project. It has also been necessary for them to master the unique chapter-writing skills required of every ma- script published in this series of laboratory monographs. With regard to gluten sensitivity we are in a period of great change, occasioned by the introduction of reproducible methods for cloning lymp- cytes, the application of physical methods to identify gluten sequences as T-cell antigens, the study of peptide responses in vitro and in vivo by either jejunal or rectal challenge, elucidating the locations of other genes concerned in pathogenesis, or the use of elegant immunohistocytochemical and mRNA probing techniques for analyzing the finer points of the mucosal inflam- tory response to gluten.

Includes supplementary material: sn.pub/extras

Klappentext

Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world, present a collection of the state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both the novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays. Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.

Inhalt
Celiac Disease.- Genotyping Methodologies.- From Linkage to Genes.- Linkage and the Transmission Disequilibrium Test in Complex Traits.- Extraction, Separation, and Purification of Wheat Gluten Proteins and Related Proteins of Barley, Rye, and Oats.- Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry.- Synthetic Peptide Libraries for T-Cell Epitope Identification.- Characterization of HLA-DQ-Specific Peptide-Binding Motifs.- Studies of Gliadin-Specific T-Cells in Celiac Disease.- Morphometric Analysis of Intestinal Mucosa.- Morphometry of Rectal Mucosa.- Organ Culture of Rectal Mucosa.- In Situ Hybridization.- Measurements of Cytokine mRNA Expression by Quantitative Polymerase Chain Reaction in Studies of Celiac Disease.- Immunohistochemistry in Research and Diagnosis of Celiac Disease.- Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease.- Antiendomysial and Antigliadin Antibody Tests and Diagnosis of Celiac Disease.- Whole Gut Lavage Fluid Analysis.