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This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding th... Weiterlesen
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This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics and function of cardiomyocytes. The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and current, Cardiomyocytes: Methods and Protocols explores complimentary areas of cardiomyocyte science that, taken together, can inform individuals with a broad range of interests.


1. Generating Primary Cultures of Murine Cardiac Myocytes and Cardiac Fibroblasts to Study Viral Myocarditis

Barbara Sherry

2. Enrichment of Cardiomyocytes in Primary Cultures of Murine Neonatal Hearts

Sreejit Parameswaran, Rajalakshmi Santhakumar, Prasanna Vidyasekar, and Rama S. Verma

3. Deep Sequencing of Cardiac MicroRNA-mRNA Interactomes in Clinical and Experimental Cardiomyopathy

Scot J. Matkovich and Gerald W. Dorn, II

4. Next Generation Sequencing Technology in the Genetics of Cardiovascular Disease

Cecilia Vecoli

5. Computational Cardiac Electrophysiology: Implementing Mathematical Models of Cardiomyocytes to Simulate Action Potentials of the Heart

Michael M. Bell and Elizabeth M. Cherry

6. Methods of Myofibrillogenesis Modeling

Nancy K. Drew and Anna Grosberg

7. Using the Mechanical Bidomain Model to Analyze the Biomechanical Behavior of Cardiomyocytes

Bradley J. Roth

8. Fabrication of a Myocardial Patch with Cells Differentiated from Human Induced-Pluripotent Stem Cells

Lei Ye, Joydeep Basu, and Jianyi Zhang

9. Efficient Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells with Growth Factors

Rajneesh Jha, Ren-He Xu, and Chunhui Xu

10. Isolation, Culturing, and Characterization of Cardiac Muscle Cells from Non-Human Primate Heart Tissue

Steven M. Hoynowski and John W. Ludlow

11. Mouse Embryonic Stem Cell-Derived Cardiac Myocytes in a Cell Culture Dish

Carley Glass, Reetu Singla, Anshu Arora, and Dinender K. Singla

12. Cryopreservation of Neonatal Cardiomyocytes

Adam C. Vandergriff, M. Taylor Hensley, and Ke Cheng

13. Evaluation of Sarcomeric Organization in Human Pluripotent Stem Cell-Derived Cardiomyocytes

Chrishan J.A. Ramachandra and Winston Shim

14. Electrotonic Coupled Metabolic Purification of Chick Cardiomyocytes

Winston Shim, Haiyang Yu, K.P. Myu Mai Ja, Muhammad Parasuram, Kee Pah Lim, and Philip Wong

15. Gene Transfer into Cardiac Myocytes

Sarah E. Lang and Margaret V. Westfall

16. Analysis of 4D Myocardial Wall Motion During Early Stages of Chick Heart Development

Madeline Midgett and Sandra Rugonyi


Titel: Cardiomyocytes
Untertitel: Methods and Protocols
EAN: 9781493947546
ISBN: 978-1-4939-4754-6
Format: Kartonierter Einband
Genre: Biologie
Anzahl Seiten: 216
Gewicht: 0g
Größe: H235mm x B235mm x T155mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2015



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