Cardiomyocytes

This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics and function of cardiomyocytes. The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and current, Cardiomyocytes: Methods and Protocols explores complimentary areas of cardiomyocyte science that, taken together, can inform individuals with a broad range of interests.

Includes cutting-edge methods and protocols in cardiomyocyte research Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Generating Primary Cultures of Murine Cardiac Myocytes and Cardiac Fibroblasts to Study Viral Myocarditis.- Enrichment of Cardiomyocytes in Primary Cultures of Murine Neonatal Hearts.- Deep Sequencing of Cardiac MicroRNA-mRNA Interactomes in Clinical and Experimental Cardiomyopathy.- Next Generation Sequencing Technology in the Genetics of Cardiovascular Disease.- Computational Cardiac Electrophysiology: Implementing Mathematical Models of Cardiomyocytes to Simulate Action Potentials of the Heart.- Methods of Myofibrillogenesis Modeling.- Using the Mechanical Bidomain Model to Analyze the Biomechanical Behavior of Cardiomyocytes.- Fabrication of a Myocardial Patch with Cells Differentiated from Human Induced-Pluripotent Stem Cells.- Efficient Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells with Growth Factors.- Isolation, Culturing, and Characterization of Cardiac Muscle Cells from Non-Human Primate Heart Tissue.- Mouse Embryonic Stem Cell-Derived Cardiac Myocytes in a Cell Culture Dish.- Cryopreservation of Neonatal Cardiomyocytes.- Evaluation of Sarcomeric Organization in Human Pluripotent Stem Cell-Derived Cardiomyocytes.- Electrotonic Coupled Metabolic Purification of Chick Cardiomyocytes.- Gene Transfer into Cardiac Myocytes.- Analysis of 4D Myocardial Wall Motion During Early Stages of Chick Heart Development.