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Capillary Electrophoresis of Nucleic Acids

  • Fester Einband
  • 484 Seiten
The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplici... Weiterlesen
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The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.


Part I. Analytical Considerations for Capillary Electrophoresis. Overview: The Application of Capillary Electrophoresis for DNA Polymorphism Analysis, Keith R. Mitchelson. DNA Separation Mechanisms During Capillary Electrophoresis, Gary W. Slater, Claude Desruisseaux, and Sylvain J. Hubert. Purification Methods for Preparing Polymerase Chain Reaction Products for Capillary Electrophoresis Analysis, Joseph M. Devaney and Michael A. Marino. Quantitative Measurements, Bruce McCord. Microchip-Based Capillary Electrophoresis Systems, Wenzhang Xie, Rong Yang, Junquan Xu, Liang Zhang, Wanli Xing, and Jing Cheng. DNA Sequencing by Capillary Array Electrophoresis, Norman J. Dovichi and JianZhong Zhang. Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing Fragments by Capillary Electrophoresis, John Bashkin. Part II. Capillary Matrix: Considerations for Capillary Electrophoresis. Influence of Polymer Concentration and Polymer Composition on Capillary Electrophoresis of DNA, Christoph Heller. Capillary Coatings: Choices for Capillary Electrophoresis of DNA, Marcella Chiari and Marina Cretich. Replaceable Polymers for DNA Sequencing by Capillary Electrophoresis, Mark A. Quesada and Steve Menchen. Sieving Matrix Selection, Tim Wehr, Mingde Zhu, and David T. Mao. Parameters Affecting Capillary Electrophoretic Separation of DNA, Haleem J. Issaq. Part III. Electrophoresis Matrix: Applications for DNA Fragment Sizing. DNA Separation by Capillary Electrophoresis in Lyotropic Polymer Liquid Crystals, Yingjie Liu and Randolph L. Rill. Capillary Electrophoresis of DNA Fragments Using Poly(Ethylene Oxide) as a Sieving Material, Yongseong Kim and Edward S. Yeung. DNA Capillary Electrophoresis Using Block Copolymer as a New Separation Medium, Benjamin Chu, Tianbo Liu, Chunhung Wu, and Dehai Liang. DNA Analysis Under Highly Denaturing Conditions in Bare Fused Silica Capillaries, Karel Kleprnk, Zdenka Mal, and Petr Bocek. Capillary Electrophoresis with Glycerol as anAdditive, Keith R. Mitchelson and Jing Cheng. Capillary Electrophoresis of DNA Fragments with Replaceable Low-Gelling Agarose Gels, Anders K. Palm. Part IV. Practical Applications for Large DNA Fragment Sizing and Fragment Collection. Robust Field Inversion Capillary Electrophoretic Separation of Long DNA Fragments, Christoph Heller, Soffia Magnsdttir, and Jean-Louis Viovy. Pulsed-Field Capillary Electrophoresis Separation of Large DNA Fragments, Michael D. Morris, Jeffrey J. Schwinefus and Olivia de Carmejane. Collection of Capillary Electrophoresis Fractions on a Moving Membrane, Soffia Magnsdttir, Christoph Heller, Phillipe Sergot, and Jean-Louis Viovy. Separation of Supercoiled DNA Using Capillary Electrophoresis, David T. Mao and Roy M. A. Lautamo. Part V. Practical Applications for Oligonucleotide and Nucleotide Analysis. Quality Control of Nucleotides and Primers for PCR, Martin J. Pearce and Nigel D. Watson. Analysis of Modified Oligonucleotides with Capillary Gel Electrophoresis, Lawrence A. DeDionisio. Use of Capillary Electrophoresis for Concentration Analysis of Phosphorothioate Oligonucleotides, G. Susan Srivatsa, Roya Pourmand, and Steven Winters. Capillary Electrophoresis Separation of Ribonucleosides, Dan-Ke Xu and Hong-Yuan Chen. On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Capillaries, Iris Barm and Gerard J. M. Bruin. Part VI. Practical Applications for Analysis of Environmental Damage and Cellular Metabolism of DNA. Capillary Electrophoresis: Mass Spectrometric Analysis of DNA Adducts, Lisa A. Marzilli, Cheryl Koertje, and Paul Vouros. Analysis of Environment-Induced DNA Damage by Capillary Electrophoresis, Zeena E. Nackerdien, Barbara A. Siles, Stacey A. Nevins, and Donald H. Atha. Assay for DNA Damage Using Immunochemical Recognition and Capillary Electrophoresis, James Z. Xing, Trevor Carnelley, Jane Lee, William P. Watson, Ewan Booth, Michael Weinfeld, and X. Chris Le. Analysis


Titel: Capillary Electrophoresis of Nucleic Acids
EAN: 9780896037793
ISBN: 978-0-89603-779-3
Format: Fester Einband
Genre: Biologie
Anzahl Seiten: 484
Gewicht: 2g
Größe: H229mm x B229mm x T152mm
Jahr: 2000
Auflage: 2001.