Cancer Genomics and Proteomics: Methods and Protocols, Second Edition includes methods for the analyses of cancer genome and proteome that have illuminated us about the changes in cancer cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cancer Genomics and Proteomics: Methods and Protocols, Second Edition seeks to aid scientists in the further study into various aspects of tumor initiation and progression.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Klappentext

Cancer Genomics and Proteomics: Methods and Protocols, Second Edition includes methods for the analyses of cancer genome and proteome that have illuminated us about the changes in cancer cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cancer Genomics and Proteomics: Methods and Protocols, Second Edition seeks to aid scientists in the further study into various aspects of tumor initiation and progression.

Inhalt

Genome-Wide Mapping Of RNA Pol-II Promoter Usage In Mouse Tissues By Chip-Seq.- Using Native Chromatin Immunoprecipitation To Interrogate Histone Variant Protein Deposition In Embryonic Stem Cells.- Reduced Representation Bisulfite Sequencing To Identify Global Alteration In The DNA Methylation.- A High-Throughput Microrna Expression Profiling System.- Using Pooled Mir30-Shrna Library For Cancer Lethal And Synthetic Lethal Screens.- Genome-Wide RNA Interference Screening To Identify Regulators Of Epigenetic Silencing Of A Tumor Suppressor Gene.- A Diphtheria Toxin Negative Selection In RNA Interference Screening.- Cancer Metabolism: Crosstalk Between Signaling And O-Glcnacylation.- Targeted Genome Modification Via Triple Helix Formation.- Synthesis Of Stabilized Alpha-Helical Peptides.- Arginine-Grafted Biodegradable Polymer: A Versatile Transfection Reagent For Both DNA And Sirna.- Using Laco Arrays To Monitor DNA Double-Strand Break Dynamics In Live Schizosaccharomyces Pombe Cells.- Zebrafish As A Platform To Study Tumor Progression.- Clonal Screens To Find Modifiers Of Partially Penetrant Phenotypes In C. Elegans.- Serum Profiling Using Protein Microarrays To Identify Disease Related Antigens.- Interrogation Of In Vivo Protein-Protein Interactions Using Transgenic Mouse Models And Stable Isotope Labeling.- New Biophysical Methods To Study The Membrane Activity Of Bcl-2 Proteins.- Purification Of Recombinant 2XMBP Tagged Human Proteins From Human Cells.- Computational Analysis In Cancer Exome Sequencing.- Matrix Factorization Methods For Integrative Cancer Genomics.- Computational Methods For DNA Copy-Number Analysis Of Tumors.