This fully updated edition provides the most recent advances in cancer cell signaling knowledge combined with some discussion of the current challenges and prospects in cancer therapy. Beginning with an important section on cancer resistance, the main obstacle to effective cancer therapy, the book continues with chapters exploring state-of-the-art methods in epigenetic control of cancer, metastasis promotion, as well as a variety of new technologies in the study of cancer. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and timely, Cancer Cell Signaling: Methods and Protocols, Third Edition serves as an ideal guide to researchers seeking to overcome the challenges in the vital field of cancer cell signaling.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt
Fighting Cancer Resistance: An Overview.- Prostate Cancer Spheroids: A Three-Dimensional Model for Studying Tumor Heterogeneity.- Enrichment and Transcriptional Characterization of Stem Cells Isolated from Human Glioblastoma Cell Lines.- Reverse Docking for the Identification of Molecular Targets of Anticancer Compounds.- Mouse Model for Efficient Simultaneous Targeting of Glycolysis, Glutaminolysis, and De Novo Synthesis of Fatty Acids in Colon Cancer.- Developing a Portable Device for the Identification of miRNAs in Fluids.- Methods for the Study of Long Non-Coding RNA in Cancer Cell Signaling.- RNA-Sequencing Analysis Pipeline for Prognostic Marker Identification in Cancer.- Correlation of Circulating Tumor Cell Measurements with 3D Quantitative Tumor Characterization to Predict Clinical Outcomes in Cancer.- Extracellular Vesicles and Their Roles in Cancer Progression.- Analysis of Tumor-Derived Exosomes by Nanoscale Flow Cytometry.- In Vitro Models for Studying Tumor Progression.-Assessment of Cell Cycle in Primitive Chronic Myeloid Leukemia Cells by Flow Cytometry after Co-Culture with Endothelial Cells.- Chimeric Antigen Receptor (CAR) T-Cell Therapy for Cancer: Challenges and Opportunities.- Cell-Internalization SELEX of RNA Aptamers as a Starting Point for Prostate Cancer Research.- Generation of Functional Genetic Study Models in Zebrafish Using CRISPR-Cas9.- Developing a Model for a siRNA Delivery System by Cancer Implantation into Zebrafish Circulation.- Fabrication of Adhesive Substrate for Incorporating Hydrogels to Investigate the Influence of Stiffness on Cancer Cell Behavior.