Cancer Cell Culture

Expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the co-culture of different cell types; and the detection and treatment of contamination. The growing of cancer cells in the laboratory has been a powerful tool for studying and understanding the biology of cancer and the effects of drugs on cancer cells. In Cancer Cell Culture: Methods and Protocols, expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. Suitable for novice and experienced researchers alike, these readily reproducible techniques solve a diverse range of experimental problems. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the coculture of different cell types; and the detection and treatment of contamination. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Cancer Cell Culture: Methods and Protocols provides both basic scientists and clinical researchers with a gold-standard collection of advanced techniques for culturing cancer cells effectively in their laboratories.

Inhalt

Part I. Introduction to Cancer Cell Culture Basic Principles of Cancer Cell Culture Simon P. Langdon Essential Techniques of Cancer Cell Culture Kenneth G. Macleod and Simon P. Langdon Part II. Characterization and Authentication Characterization and Authentication of Cancer Cell Lines: An Overview Simon P. Langdon Authentication of Cancer Cell Lines by DNA Fingerprinting Wilhelm G. Dirks and Hans G. Drexler Cytogenetic Characterization of Tumor Cell Lines Roderick A. F. MacLeod and Hans G. Drexler Part III. Isolation and Culture of Specialized Cell Types Isolation and Culture of Colon Cancer Cell Lines Jae-Gahb Park, Ja-Lok Ku, and So-Yeon Park Isolation and Culture of Melanoma Cell Lines Adam I. Riker Isolation and Culture of Human Brain Tumor Cells German G. Gomez and Carol A. Kruse Isolation and Culture of Renal Cancer Cell Lines Jae-Gahb Park, Ja-Lok Ku, and So-Yeon Park Isolation and Culture of Prostate Cancer Cell Lines Nora M. Navone, Matilde Olive, and Patricia Troncoso Isolation and Culture of Ovarian Cancer Cell Lines Simon P. Langdon Isolation and Culture of Leukemia Cell Lines Hans G. Drexler Part IV. Functional Assays in Cell Culture Cell Sensitivity Assays: Clonogenic Assay Jane A. Plumb Cell Sensitivity Assays: The MTT Assay Jane A. Plumb PARP Cleavage as a Means of Assessing Apoptosis Peter Mullen Detection of Apoptosis by the TUNEL Assay John Lawry Apoptosis Measurement by Annexin V Staining Eric Miller Cell Adhesion Assays William N. Scott Radial Monolayer Cell Migration Assay Michael E. Berens and Christian Beaudry Invasion and Motility Assays William N. Scott Identification of Senescence in Cancer Cells Lesley J. Jardine Angiogenesis Assays William N. Scott Flow Cytometric DNA Analysis of Human Cancer Cell Lines Peter Mullen Part V. Modification of Cancer Cells DNA-Mediated Gene Transfer Amanda J. M. O'Donnell Development of Drug-Resistant Models Helen M. Coley Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene Norman J. Maitland, Catherine A. Macintosh, Christian Schmitz, and Shona H. Lang The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts Peter Mullen Part VI. Coculture Systems Coculture of Prostate Cancer Cells Shona H. Lang and Norman J. Maitland Coculture of Ovarian Cells Using Porous Tissue Culture Inserts Katherine E. Snow and Simon P. Langdon Part VII. Cell Line Contamination Cell Culture Contamination: An Overview Simon P. Langdon Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction Cord C. Uphoff and Hans G. Drexler Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics Cord C. Uphoff and Hans G. Drexler Appendix 1: Formulations of Commonly Used Cell Culture Media Appendix 2: Human Cancer Cell Lines Available from the ATCC and DSMZ Cell Banks (Combined List) Index