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Calcium-Binding Protein Protocols

  • Fester Einband
  • 415 Seiten
Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of p... Weiterlesen
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Beschreibung

Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on-off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

Zusammenfassung
The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

Inhalt

II. Methods to Study Calcium-Binding Proteins Quantitative Analysis of Ca2+ Binding by Flow Dialysis Michio Yazawa Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators Sara Linse Deconvolution of Calcium Binding Curves: Facts and Fiction Jacques Haiech and Marie-Claude Kilhoffer Absorption and Circular Dichroism Spectroscopy Stephen R. Martin and Peter M. Bayley Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins Heinz Fabian and Hans J. Vogel Steady State Fluorescence Spectroscopy Aalim M. Weljie and Hans J. Vogel Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins J. David Johnson and Svetlana B. Tikunova Surface Plasmon Resonance of Calcium-Binding Proteins Karin Julenius Differential Scanning Calorimetry Maria M. Lopez and George I. Makhatadze Isothermal Titration Calorimetry Maria M. Lopez and George I. Makhatadze Multiangle Laser Light Scattering and Sedimentation Equilibrium Leslie D. Hicks, Jean-René Alattia, Mitsuhiko Ikura, and Cyril M. Kay Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in Their Interactions with Regulatory Targets Jill Trewhella and Joanna K. Krueger Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry Amanda L. Doherty-Kirby and Gilles A. Lajoie Synthetic Calcium-Binding Peptides Gary S. Shaw Proteolytic Fragments of Calcium-Binding Proteins Richard D. Brokx and Hans J. Vogel Electron Magnetic Resonance Studies of Calcium-Binding Proteins Lawrence J. Berliner Cadmium-113 and Lead-207 NMR of Calcium-Binding Proteins Teresa E. Clarke and Hans J. Vogel Calcium-43 of NMR ofCalcium-Binding Proteins Torbjörn Drakenberg Exploring Familial Relationships Using Multiple Sequence Alignment Aalim M. Weljie and Jaap Heringa Structure Determination by NMR - Isotope Labeling Monica X. Li, David C. Corson, and Brian D. Sykes Protein Structure Calculation from NMR Data Tapas K. Mal, Stefan Bagby, and Mitsuhiko Ikura Shape and Dynamics of Calcium Binding Proteins Investigated by Nitrogen-15 NMR Relaxation Jörn M. Werner, Iain D. Campbell, and A. Kristina Downing The Use of Dipolar Couplings for the NMR Structure Refinement of a Pair of Calcium Binding EGF Domains Jonathan Boyd, Iain D. Campbell, and A. Kristina Downing Vector Geometry Mapping: A Method to Characterise the Conformation of Helix-Loop-Helix Calcium Binding Proteins Kyoko L. Yap, James B. Ames, Mark B. Swindells, and Mitsuhiko Ikura Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography Ryoji Kobayashi Enzymatic Assays to Compare Calmodulin Isoforms, Mutants and Chimeras Michael P. Walsh, Jacquelyn E. Van Lierop, Cindy Sutherland, Ritsu Kondo, and J. David Johnson Gene Expression in Transfected Cells Kate Hughes, Juha Saarikettu, and Thomas Grundström Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins Anthony Persechini Studying the Spatial Distribution of Ca2+-Binding Proteins: How Does it Work for Calmodulin? Katalin Török, Richard Thorogate, and Steven Howell

Produktinformationen

Titel: Calcium-Binding Protein Protocols
Untertitel: Volume 2: Methods and Techniques
Editor:
EAN: 9780896036895
ISBN: 978-0-89603-689-5
Format: Fester Einband
Genre: Biologie
Anzahl Seiten: 415
Gewicht: 807g
Größe: H229mm x B229mm x T152mm
Jahr: 2002
Auflage: 2002.
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