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A Guide to Protein Isolation

  • Fester Einband
  • 249 Seiten
It is a truism of science that the more fundamental the subject, the more universally applicable it is. Neverthelens, it is import... Weiterlesen
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Beschreibung

It is a truism of science that the more fundamental the subject, the more universally applicable it is. Neverthelens, it is important to strike a level of "fundamentalness" appropriate to the task in hand. For example, an in-depth study of the mechanics of motor cars would tell one nothing about the dynamics of traffic. Traffic exists on a different "level" - it is dependent upon the existence of motor vehicles but the physics and mathematics of traffic can be adequately addressed by considering motor vehicles as mobile "blobs", with no consideration of how they become mobile. To start a discourse on traffic with a consideration of the mechanics of motor vehicles would thus be inappropropriate. In writing this volume, I have wrestled with the question of the appropriate level at which to address the physics underlying many of the techniques used in protein isolation. I have tried to strike a level as would be used by a mechanic (with perhaps a slight leaning towards an engineer) - i.e. a practical level, offering appropriate insight but with minimal mathematics. Some people involved in biochemical research have a minimal grounding in chemistry and physics and so I have tried to keep it as simple as possible.

Zusammenfassung
Traffic exists on a different "level" - it is dependent upon the existence of motor vehicles but the physics and mathematics of traffic can be adequately addressed by considering motor vehicles as mobile "blobs", with no consideration of how they become mobile.

Inhalt

1 Basic physical concepts applicable to the isolation of proteins.- 2 An overview of protein isolation.- 3 Assay, extraction and subcellular fractionation.- 4 Concentration of the extract.- 5 Chromatography.- 6 Electrophoresis.- 7 Immunological methods.- 8 Some common practical methods.- Answers to study questions.- 1.- 2.- 3.- 4.- 5.- 6.- 7.- Further sources of information.

Produktinformationen

Titel: A Guide to Protein Isolation
Autor:
EAN: 9781402012242
ISBN: 978-1-4020-1224-2
Format: Fester Einband
Genre: Biologie
Anzahl Seiten: 249
Gewicht: 562g
Größe: H240mm x B240mm x T160mm
Jahr: 2003
Auflage: 2nd edition
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