Biosensors and Biodetection

Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. This book describes the major technologies in the field in extensive technical detail, providing a user-friendly guide to this vital technology.

Unique, one-of-a-kind laboratory manual for detailed, standardized protocols on biosensors or lab-on-a-chip technology Describes basic types and the basic elements of biosensors from an experimental point of view Provides multiple experimental approaches to enable readers to choose an appropriate design and to combine elements from different designs Includes enough technical information to promote understanding of the technology and the ability to conduct similar experiments

Inhalt
Mechanical Detectors.- A Set of Piezoelectric Biosensors Using Cholinesterases.- Piezoelectric Biosensors for AptamerProtein Interaction.- Piezoelectric Quartz Crystal Resonators Applied for Immunosensing and Affinity Interaction Studies.- Biosensors Based on Cantilevers.- Piezoelectric-Excited Millimeter-Sized Cantilever Biosensors.- Electrochemical Detectors.- Preparation of Screen-Printed Electrochemical Immunosensors for Estradiol, and Their Application in Biological Fluids.- Electrochemical DNA Biosensors: Protocols for Intercalator-Based Detection of Hybridization in Solution and at the Surface.- Electrochemical Biosensor Technology: Application to Pesticide Detection.- Electrochemical Detection of DNA Hybridization Using Micro and Nanoparticles.- Electrochemical Immunosensing Using Micro and Nanoparticles.- Methods for the Preparation of Electrochemical Composite Biosensors Based on Gold Nanoparticles.- Lateral Flow.- Immunochromatographic Lateral Flow Strip Tests.- Liposome-Enhanced Lateral-Flow Assays for the Sandwich-Hybridization Detection of RNA.- Rapid Prototyping of Lateral Flow Assays.- Lateral Flow Colloidal Gold-Based Immunoassay for Pesticide.- Ligands.- Synthesis of a Virus Electrode for Measurement of Prostate Specific Membrane Antigen.- In Vivo Bacteriophage Display for the Discovery of Novel Peptide-Based Tumor-Targeting Agents.- Biopanning of Phage Displayed Peptide Libraries for the Isolation of Cell-Specific Ligands.- Biosensor Detection Systems: Engineering Stable, High-Affinity Bioreceptors by Yeast Surface Display.- Antibody Affinity Optimization Using Yeast Cell Surface Display.- Using RNA Aptamers and the Proximity Ligation Assay for the Detection of Cell Surface Antigens.- In Vitro Selection of Protein-Binding DNA Aptamers as Ligandsfor Biosensing Applications.- Protein and DNA Preparation.- Immobilization of Biomolecules onto Silica and Silica-Based Surfaces for Use in Planar Array Biosensors.- Rapid DNA Amplification Using a Battery-Powered Thin-Film Resistive Thermocycler.