This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 2 delves into techniques for heterogeneous conjugates, protein fragment-complementation assays, BRET-based imaging, as well as instrumentation and software. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 2 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Quantitative Assessment of the Efficacy of Near-Infrared Photoimmunotherapy with Bioluminescence Imaging.- Evaluation of the Efficacy of Saracatinib-Loaded Nanoparticles in Lymphatic Metastases of HNSCC with the Aid of Bioluminescence Imaging.- Reactive Oxygen Species-Responsive and Self-Illuminating Nanoparticles for Inflammation and Tumor Imaging.- Antibacterial Activity Evaluation of ZnO, CuO, and TiO 2 Nanoparticles in Solution and Thin Films.- BRET-Based Dual-Color (Visible/Near-Infrared) Molecular Imaging Using a Quantum Dot/EGFP-Luciferase Conjugate.- Polyhistidine-Tag-Enabled Conjugation of Quantum Dots and Enzymes to DNA Nanostructures.- Real-Time Quantification of Cell Internalization Kinetics by Bioluminescent Probes.- Quantitative Imaging of Retinoic Acid Activities in Living Mammalian Cells.- Bioluminescence-Based Complementation Assay to Correlate Conformational Changes in Membrane-Bound Complexes with Enzymatic Function.- The NanoBiT-Based Homogenous Ligand-Receptor Binding Assay.- Monitoring Hippo Signaling Pathway Activity Using a Luciferase-Based Large Tumor Suppressor (LATS) Biosensor.- Screening of ProteinProtein Interaction Modulators Using BRETBased Technology.- TRUPATH: An Open-Source Biosensor Platform for Interrogating the GPCR Transducerome.- MERLIN: A BRET-Based Proximity Biosensor for Studying MitochondriaER Contact Sites.- Single Cell NanoBRET Imaging with Green-Range Halo-Tag Acceptor.- Method for Measuring Bioactive Molecules in Blood by a Smartphone Using Bioluminescent Ratiometric Indicators.- BRET Sensors for Imaging Membrane Integrity of Microfluidically Generated Extracellular Vesicles.- In Vivo Assessment of Protein-Protein Interactions Using BRET Assay.- Live Cell Imaging of ATP Dynamics in Plant Cells.- Bioluminescence Resonance Energy Transfer for Global DNA Methylation Quantification.- In Vivo Bioluminescent Imaging of Bone Marrow Derived-Mesenchymal Stem Cells in Mice.- In Vivo Imaging of Oxidativeand Hypoxic Stresses in Mice Model of Amyotrophic Lateral Sclerosis.- ATP Sensing Paper with Smartphone Bioluminescence-Based Detection.- Organ Bioluminescence Imaging under Machine Perfusion Setting for Assessing Quality of Harvested Organ Preservation.- Time-Lapse Bioluminescence Imaging of Hes7 Expression In Vitro and Ex Vivo.- Bioluminescence-Optogenetics: A Practical Guide.- Applications of Bioluminescence-Optogenetics in Rodent Models.- One-Channel Microsliding Luminometer for Quantifying Low Energy Bioluminescent Lights.- Compact Eight-Channel Light Sensing System for Bioassays.- Characterization of Firefly Flashes at Various Temperatures in Different Wavelength Regions.- Bioluminescent Monitoring of Circadian Rhythms in Isolated Mesophyll Cells of Arabidopsis at Single-Cell Level.- Exploring Phylogenetic Relationships and Divergence Times of Bioluminescent Species Using Genomic and Transcriptomic Data.