This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 1 delves into techniques for the establishment of luciferins and luciferases, basic in vitro and in vivo applications, as well as protocols on multiplex imaging platforms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 1 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Gene Cloning and Functional Analysis of the Luciferase from Luminous Syllids of the Genus Odontosyllis .- Synthetic Coelenterazine Derivatives and Their Application for Bioluminescence Imaging.- Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging.- Biosynthesis-Inspired Deracemizative Production of D-Luciferin In Vitro by Combining Luciferase and Thioesterase.- Production of Metridia Luciferase in Native Form by Oxidative Refolding from E. coli Inclusion Bodies.- Production of Copepod Luciferases via Baculovirus Expression System.- Molecular Tension Probe for In Vitro Bioassays.- Optimized Loop-Mediated Amplification (LAMP) Allows Single Copy Detection Using Bioluminescent Assay in Real Time (BART).- A Simple and Rapid Bioluminescence-Based Functional Assay of Organic Anion Transporter 1 as a d-Luciferin Transporter.- A Simple Bioluminescent Assay for the Screening of Cytotoxic Molecules against the Intracellular Form of Leishmania infantum.- A Simple, Robust, and Affordable Bioluminescent Assay for Drug Screening against Infective African Trypanosomes.- Imaging of Autonomous Bioluminescence Emission from Single Mammalian Cells.- Rapid Single-Cell Detection of Beer-contaminating Lactic Acid Bacteria Using Bioluminescence/Rapid Microbe Detection.- Bioluminescence of Aliivibrio fischeri in Artificial Seawater and Its Application in Fungicide Sensing.- A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor.- Design of an Intron-Retained Bioluminescence Reporter and Its Application in Imaging of Pre-mRNA Splicing in Living Subjects.- Generation of Bi-Reporter Expressing Tri-Segmented Arenavirus.- Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2.- Generation, Characterization, and Applications of Influenza A Reporter Viruses.- Optimized Aequorin Reconstitution Protocol to Visualize Calcium Ion Transients in the Heart of TransgenicZebrafish Embryos In Vivo.- Quantification and Imaging of Exosomes via Luciferase-Fused Exosome Marker Proteins: ExoLuc System.- Bioluminescent Tracking of Human Induced Pluripotent Stem Cells In Vitro and In Vivo.- Noninvasive In Vivo Tracking of Mammalian Cells Stably Expressing Firefly Luciferase.- Bioluminescence Imaging for Evaluation of Antitumor Effect In Vitro and In Vivo ** in Mice Xenografted Tumor Models.- Detection of Spontaneous Bone Metastases of Solid Human Tumor Xenografts in Mice.- In Vivo Imaging Analysis of an Inner Ear Drug Delivery in Mice: Comparison of Inner Ear Drug Concentrations Over Time.- Protocols for the Evaluation of a Lymphatic Drug Delivery System Combined with Bioluminescence to Treat Metastatic Lymph Nodes.- In Vivo Bioluminescent Imaging of Rabies Virus Infection and Evaluation of Antiviral Drug.- Imaging Infection by Vector-Borne Protozoan Parasites Using Whole-Mouse Bioluminescence.- Longitudinal Tracing of Lyssavirus Infection in Mice via InVivo Bioluminescence Imaging.- Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for the Discovery of Anti-Relapse Compounds.- Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase Reporter Plasmids.- Multiplex Hextuple Luciferase Assaying.- Molecular Imaging of Tumor Progression and Angiogenesis by Dual Bioluminescence.