Biological Small Angle Scattering: Techniques, Strategies and Tips

Includes the principles and theoretical background of various SAS techniquesStates practical aspects that range from sample preparation to data publication
Covers techniques for improving data quality and analysis, as well as different scientific applications of SAS.
Gives abundant illustrations and practical tips
Explains clearly the principles and the reviews on the latest progresses and serves as a guide through all aspects of biological solution SAS for readers

Includes the principles and theoretical background of various SAS techniques States practical aspects that range from sample preparation to data publication Covers techniques for improving data quality and analysis, as well as different scientific applications of SAS. Gives abundant illustrations and practical tips Explains clearly the principles and the reviews on the latest progresses and serves as a guide through all aspects of biological solution SAS for readers

Inhalt

Sample and Buffer preparation for SAXS.- Considerations for sample preparation using size-exclusion chromatography.- How to analyze and present SAS data for publication.- Designing and Performing Biological Solution Small-Angle Neutron Scattering Contrast Variation Experiments on Multi-component Assemblies.- SAS-based structural modeling and model validation.- Structural Characterization of Highly Flexible Proteins by Small-Angle Scattering.- What can we learn from wide-angle solution scattering?.- SAS-based studies of protein fibrillation.- High Resolution Distance Distributions Determined by X-ray and Neutron Scattering.- A successful combination: coupling SE-HPLC with SAXS.- Applications of SANS to study membrane protein systems.- Hybrid applications of solution scattering to aid structural biology.- A practical guide to iSPOT modeling: An integrative structural biology platform.- Small angle scattering for pharmaceutical applications: From drugs to drug delivery system.