This volume continues the discussion of the problems of in vivo and in vitro. The recently solved X-ray structure of the mitochondrial creatine kinase and its molecular biology cellular bioenergetics - the tradition we started in 1994 by publication of the focused issue of Molecular and Cellular are analyzed with respect to its molecular physiology and Biochemistry, volume 133/134 and a book 'Cellular Bio functional coupling to the adenine nucleotide translocase, as energetics: role of coupled creatine kinases' edited by V. Saks well as its participation, together with the adenylate kinase and R. Ventura-Clapier and published by Kluwer Publishers, system, in intracellular energy transfer. The results of the Dordrecht -Boston. In the present volume, use of quantitative studies of creatine kinase deficient transgenic mice are methods of studies of organized metabolic systems, such as summarized and analyzed by using mathematical models of mathematical modeling and Metabolic Control Analysis, for the compartmentalized energy transfer, thus combining two investigation of the problems of bioenergetics of the cell is powerful new methods of the research. All these results, described together with presentation of new experimental together with the physiological and NMR data on the cardiac results. The following central problems of the cellular bio metabolic and mitochondrial responses to work-load changes energetics are the focus of the discussions: the mechanisms concord to the concept of metabolic networks of energy of regulation of oxidative phosphorylation in the cells in vivo transfer and feedback regulation.

Klappentext

This book describes the use of quantitative methods in studies in organized metabolic systems, such as mathematical modeling and Metabolic Control Analysis, for investigation of the problems of bioenergetics of the cell and also presents new experimental results. The following central problems of cellular bioenergetics are the focus of the discussions: the mechanisms of regulation of oxidative phosphorylation in cells in vivo in health and disease, the influence of fine intracellular structural organization on mitochondrial function, the intracellular networks of energy transfer and metabolic feedback signaling, the role of mitochondria in intracellular calcium cycling, and changes in mitochondrial membrane systems and cellular energetics under pathological conditions. Systematic studies from many laboratories presented in this volume convincingly show that many mitochondrial properties, including affinities for mitochondrial creatine kinase and its molecular biology are related to its molecular physiology and functional coupling to adenine nucleotide translocase, as well as its participation, together with the adenylate kinase system, in intracellular energy transfer. The results of the studies of creatine kinase-deficient transgenic mice are summarized and analyzed using mathematical models of the compartmentalized energy transfer, thus combining two powerful new research methods. All these results, together with physiological and NMR data on the cardiac metabolic and mitochondrial responses to workload changes, confirm the concept of metabolic networks of energy transfer and feedback regulation. The papers, mostly review articles, are written by the leading experts in the field who analyze their experience of research and give insights into the future development of this increasingly important area of biological sciences.

Inhalt
What do we not bioenergetics? A general view on the state of the art.- I: Bioenergetics of mitochondria: In vitro and in vivo studies.- Top-down elasticity analysis and its application to energy metabolism in isolated mitochondria and intact cells.- A model of O·2- generation in the complex III of the electron transport chain.- Quantitative analysis of some mechanisms affecting the yield of oxidative phosphorylation: Dependence upon both fluxes and forces.- Oxidative phosphorylation in intact hepatocytes: Quantitative characterization of the mechanisms of change in efficiency and cellular consequences.- Yeast mitochondrial metabolism: From in vitro to in situ quantitative study.- Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vivo.- Cytoskeleton and mitochondrial morphology and function.- Energetics of swelling in isolated hepatocytes: A comprehensive study.- II: Energy transfer networks: Molecular physiology of kinases, lessons from transgenic mice, mathematical theories.- Functional aspects of the X-ray structure of mitochondrial creatine kinase: A molecular physiology approach.- Oligomeric state and membrane binding behaviour of creatine kinase isoenzymes: Implications for cellular function and mitochondrial structure.- Molecular characterization of the creatine kinases and some historical perspectives.- Adenylate kinase: Kinetic behavior in intact cells indicates it is integral to multiple cellular processes.- Cytoarchitectural and metabolic adaptations in muscles with mitochondrial and cytosolic creatine kinase deficiencies.- In situ measurements of creatine kinase flux by NMR. The lessons from bioengineered mice.- Mathematical model of compartmentalized energy transfer: Its use for analysis and interpretation of31P-NMR studies of isolated heart of creatine kinase deficient mice.- Functional coupling of creatine kinases in muscles: Species and tissue specificity.- Theoretical modelling of some spatial and temporal aspects of the mitochondrion/creatine kinase/myofibril system in muscle.- Quantitative studies of enzyme-substrate compartmentation, functional coupling and metabolic channelling in muscle cells.- III: Metabolic signalling and calcium: Regulation of mitochondrial oxidative phosphorylation in vivo.- Subtleties in control by metabolic channelling and enzyme organization.- The dynamic regulation of myocardial oxidative phosphorylation: Analysis of the response time of oxygen consumption.- Is it possible to predict any properties of oxidative phosphorylation in a theoretical way?.- Role of mitochondrial calcium transport in the control of substrate oxidation.- Modulation of cell calcium signals by mitochondria.- IV: Bioenergetics and medicine.- Mitochondrial function as a determinant of recovery or death in cell response to injury.- Role of cellular energetics in ischemia-reperfusion and ischemic preconditioning of myocardium.- Early ischemia-induced alterations of the outer mitochondrial membrane and the intermembrane space: A potential cause for altered energy transfer in cardiac muscle?.- Metabolic control analysis and mitochondrial pathologies.- Mechanisms of thyroid hormone control over sensitivity and maximal contractile responsiveness to ?-adrenergic agonists in atria.- Creatine supplementation in health and disease. Effects of chronic creatine ingestion in vivo: Down-regulation of the expression of creatine transporter isoforms in skeletal muscle.- Clinical cardiac magnetic resonance spectroscopypresent state and future directions.- Time-Resolved Spectroscopyof mitochondria, cells and tissues under normal and pathological conditions.- Index to Volume 184.