This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis.

Basic laboratory techniques, approved in several international molecular biology courses Step-by-step protocols with detailed practical hints, troubleshooting guide and short background information The protocols are written by authors with intensive practical experience

Klappentext

This manual introduces the reader to basic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. BREach protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

Inhalt
1 cDNA Synthesis and Cloning.- 2 DNA Sequencing.- 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- 4 PCR-Based Site-Specific Mutagenesis.- 5 Analysis of Specific Protein-DNA Interactions.- 6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- 7 Immunological Methods for Analysis of Recombinant Proteins.- 8 Protein Synthesis in Cell-Free Systems.