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Baculovirus and Insect Cell Expression Protocols

  • Kartonierter Einband
  • 520 Seiten
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The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters in... Weiterlesen
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The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts


PART I. Introduction

1 Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior

David W. Murhammer

PART II. Baculovirus Molecular Biology/Development of Recombinant Baculoviruses

2 Introduction to Baculovirus Molecular Biology

Barbara J. Kelly, Linda A. King And Robert D. Possee

3 Baculovirus Transfer Vectors

Robert D. Possee And Linda A. King

4 Recombinant Baculovirus Isolation

Linda A. King, Richard Hitchman And Robert D. Possee

5 Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System

James A. Fornwald, Quinn Lu, Frederick M. Boyce And Robert S. Ames


6 Available Lepidopteran Insect Cell Lines

Dwight E. Lynn and Robert E. Harrison

7 Lepidopteran Insect Cell Line Isolation from Insect Tissue

Dwight E. Lynn

8 Development Of Serum-Free Media For Lepidopteran Insect Cell Lines

Leslie C. L. Chan and Steven Reid

9 Routine Maintenance And Storage Of Lepidopteran Insect Cell Lines And Baculoviruses

Dwight E. Lynn and Robert L. Harrison

PART IV. Protein Production with Recombinant Baculoviruses

10 Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System

Jian-Ping Yang

11 Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System

Christine M. Thompson, Johnny Montes, Marc G. Aucoin and Amine A. Kamen

12 Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors

Sue H. Kadwell and Laurie K. Overton

13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae

Elena Kovaleva and David C. Davis

14 Production Of Virus-Like Particles For Vaccination

Christine M. Thompson, Marc G. Aucoin and Amine A. Kamen

15 Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells

Stephanie Chen

PART V. Recombinant Protein Production with Transformed Insect Cells

16 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression

Robert L. Harrison and Donald L. Jarvis

17 Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression

Marija Backovic and Thomas Krey

18 Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression

Robert L. Harrison and Donald L. Jarvis

PART VI Baculovirus Development and Production for Use as Insecticides

19Introduction To The Use Of Baculoviruses As Biological Insecticides

Holly J. R. Popham, Tyasning Nusawardani And Bryony C. Bonning

20 Baculovirus Insecticide Production In Insect Larvae

Nikolai Van Beek and David C. Davis

21Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses

Holly J. R. Popham, Mark R. Ellersieck, Huarong Li and Bryony C. Bonning

PART VII. Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System

22 Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants

Hsuan-Chen Wu, Hyung Joon Cha and William E. Bentley

23 Tubular Bioreactor for Probing Baculovirus Infection and Protein Production

Hsuan-Chen Wu, Yu-Chen Hu and William E. Bentley

24Gene Silencing In Insect Cells Using Rnai

Hsuan-Chen Wu, John C. March and William E. Bentley

25 Using the Baculovirus/Insect Cell System to Study Apoptosis

Nor Chejanovsky

26 Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells

Christian Hofmann


Titel: Baculovirus and Insect Cell Expression Protocols
EAN: 9781493979783
ISBN: 1493979787
Format: Kartonierter Einband
Herausgeber: Springer New York
Anzahl Seiten: 520
Gewicht: 967g
Größe: H254mm x B178mm x T27mm
Jahr: 2018
Auflage: Softcover reprint of the original 3rd ed. 2016

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