In this book, in-depth methods and state-of-the-art protocols are presented for investigating specific mechanisms of pathogenesis for a wide range of bacteria. This invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections. Up-to-date molecular typing methods for Staphylococcus aureus and a new model of streptococcal pharyngitis in non-human primates are also included. This book will prove an invaluable collection for microbiologists, immunologists, cell biologists and infectious disease clinicians - and indispensable to all science researchers interested in studying pathogenic bacteria and related disease processes.

Collects time-tested, reproducible laboratory protocols, including tricks and hints, written by experts in the field Investigates specific mechanisms of pathogenesis for a wide range of bacteria Includes novel approaches for identifying therapeutic targets designed to control infections

Inhalt
Preface.- Contributors.- List of Color Plates.- I: Mechanisms of Bacterial Pathogenesis.- 1 Isolation and Characterization of LipopolysaccharidesMichael A. Apicella.- 2 Proteomic Analysis of Proteins Secreted by Streptococcus pyogenesMichelle A. Chaussee, Emily J. McDowell, and Michael S. Chaussee.- 3 Proteomic Analysis to Investigate Regulatory Networks in Staphylococcus aureusSusanne Engelmann and Michael Hecker.- 4 Microarray Comparative Genomic Hybridization for the Analysis of Bacterial Population Genetics and EvolutionCaitriona M. Guinane and J. Ross Fitzgerald.- 5 Detection and Inhibition of Bacterial CellCell CommunicationScott A. Rice, Diane McDougald, Michael Givskov, and Staffan Kjelleberg.- 6 A System for Site-Specific Genetic Manipulation of the Relapsing Fever Spirochete Borrelia hermsiiJames M. Battisti, Sandra J. Raffel, and Tom G. Schwan.- 7 Transposon Mutagenesis of the Lyme Disease Agent Borrelia burgdorferiPhilip E. Stewart and Patricia A. Rosa.- 8 The Biofilm Exopolysaccharide Polysaccharide Intercellular Adhesin?A Molecular and Biochemical ApproachCuong Vuong and Michael Otto.- II: Host-Pathogen Interaction.- 9 Analysis of Staphylococcus aureus Gene Expression During PMN PhagocytosisJovanka M. Voyich, Dan E. Sturdevant, and Frank R. DeLeo.- 10 Examining the VectorHostPathogen Interface With Quantitative Molecular ToolsJason E. Comer, Ellen A. Lorange, and B. Joseph Hinnebusch.- 11 Intracellular Localization of Brucella abortus and Francisella tularensis in Primary Murine MacrophagesJean Celli.- 12 Rate and Extent of Helicobacter pylori PhagocytosisLee-Ann H. Allen.- 13 Culture, Isolation, and Labeling of Anaplasma phagocytophilum for Subsequent Infection of Human NeutrophilsDori L. Borjesson.- 14 Ultrastructural Analysis ofBacteriaHost Cell InteractionsDavid W. Dorward.- 15 Infection of Human Monocyte-Derived Macrophages With Coxiella burnetiiJeffrey G. Shannon and Robert A. Heinzen.- 16 Infection of Epithelial Cells With Salmonella entericaOlivia Steele-Mortimer.- 17 Determining the Cellular Targets of Reactive Oxygen Species in Borrelia burgdorferiJulie A. Boylan and Frank C. Gherardini.- III: Animal Models of Bacterial Infection.- 18 Bioluminescent Imaging of Bacterial Biofilm Infections In VivoJagath L. Kadurugamuwa and Kevin P. Francis.- 19 The Cotton Rat as a Model for Staphylococcus aureus Nasal Colonization in Humans: Cotton Rat S. aureus Nasal Colonization ModelJohn F. Kokai-Kun.- 20 A Non-Human Primate Model of Acute Group A Streptococcus PharyngitisPaul Sumby, Anne H. Tart, and James M. Musser.- IV: Identification of Therapeutic Targets and Typing Methods.- 21 Target-Based Antimicrobial Drug DiscoveryLefa E. Alksne and Paul M. Dunman.- 21 Sequence Analysis of the Variable Number Tandem Repeat in Staphylococcus aureus Protein A Gene: spa TypingBarun Mathema, Jose Mediavilla, and Barry N. Kreiswirth.