Focusing on how best to study bacterial adhesion, this volume helps bridge the communication divide between biologists, chemists and physics researchers and aims at stimulating fresh and groundbreaking research into this essential function of bacteria.

Over the last few years, bacterial adhesion has become a more and more important and active scientific area, but the field lacks communication and scientific exchange between medical and microbiology researchers who work with the relevant biological systems, and biochemists, structural biologists and physicists, who know and understand the physical methods best suited to investigate the phenomenon at the molecular level. The field consequently would benefit from a cross-disciplinary conference enabling such communication. This book tries to bridge the gap between the disciplines.

Includes methods from such diverse fields as microbiology, structural biology, biophysics and biochemistry Has additional color figures in the online (PDF) version Displays the principles of bacterial adhesion over a broad range of medically relevant species Provides a broad overview of adhesion from multiple perspectives, ranging from biophysics to medical applications Useful for clinical researchers who want to gain a broad understanding of the science behind nosocomial infections

Klappentext
Adhesion plays a major role in the bacterial lifestyle. Bacteria can adhere to organic and inorganic surfaces, to each other, and of course to host cells during pathogenesis. The focus of this book is: how are such adhesion phenomena best studied? Microbial genetics experiments have greatly enhanced our knowledge of what bacterial factors are involved in adhesion. For numerous reasons, though, biochemical and structural biology knowledge of the molecular interactions involved in adhesion are limited. One major problem has been a lack of interdisciplinary research and understanding in the field. On the one hand, the microbiologists lack detailed knowledge of the biophysical possibilities and have limited access to the frequently expensive instrumentation involved while on the other hand, the experts in these methods frequently do not have access to the biological materials, nor do they necessarily understand the biological questions to be answered. The purpose of this book is thus to overcome this gap in communication between researchers in biology, chemistry and physics and to display the many ways and means to investigate bacterial adhesion. We hope to stimulate new and ground-breaking research.

Inhalt

1. Adhesins of human pathogens from the genus Yersinia; 2. Adhesive mechanisms of Salmonella enteric; 3. ** Adhesion Mechanisms of Borrelia burgdorferi; 4. Adhesins of Bartonella spp.; 5. Adhesion Mechanisms of Plant-Pathogenic Xanthomonadeae; 6. Adhesion by Pathogenic Corynebacteria; 7. Adhesion Mechanisms of Staphylococci; 8. Protein Folding in Bacterial Adhesion: Secretion and Folding of Classical Monomeric Autotransporters; 9. Structure and Biology of Trimeric Autotransporter Adhesins; 10. Crystallography and EM of chaperone/usher pilus systems; ** 11. Crystallography of Gram-positive Bacterial Adhesins; ** 12. The nonideal coiled coil of M protein and its multifarious functions in pathogenesis; 13. Bacterial Extracellular Polysaccharides; 14. Carbohydrate mediated bacterial adhesion; ** 15. The Application of NMR Techniques to Bacterial Adhesins; ** 16. Electron microscopy techniques to study bacterial adhesion; ** 17. EM Reconstruction of Adhesins: ** 18. Atomic force microscopy to study intermolecular forces and bonds associated with bacteria; 19. Assessing Bacterial Adhesion on an Individual Adhesin and Single Pili Level using Optical Tweezers; 20. Short time-scale bacterial adhesion dynamics; 21. Deciphering Biofilm Structure and Reactivity by Multiscale Time-resolved Fluorescence Analysis; 22. Inhibition of Bacterial Adhesion on Medical Devices.