This volume covers a wide range of model systems from invertebrate to humans and in vitro and in vivo . Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Axonal Transport: Methods and Protocols aims to further our understanding of the trafficking of cellular components in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of the neuron.

The chapters Live imaging of RNA Transport and Translation in Xenopus Retinal Axons, Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons, Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons, Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport, and In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

The Giant Axon of the Squid: A Simple System for Axonal Transport Studies.- Live Imaging and Quantitative Analysis of Organelle Transport In Sensory Neurons of Aplysia Californica.- Live imaging of RNA Transport and Translation in Xenopus Retinal Axons.- Imaging Axonal Transport in Ex Vivo Central and Peripheral Nerves.- In vivo Imaging of Axonal Organelle Transport in the Mouse Brain.- Studying Axonal Transport in the Brain by Manganese-Enhanced Magnetic Resonance Imaging (MEMRI).- Microfluidic Neuromuscular Co-Culture System for Tracking Cell-To-Cell Transfer and Axonal Transport of Labeled Proteins.- Imaging Diversity in Slow Axonal Transport.- Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses.- Molecular Analysis of Axonal Transport Dynamics Upon Modulation of Microtubule Acetylation.- Live-cell Imaging of RNA Transport In Axons Of Cultured Primary Neurons.- Visualizing Vesicle-Bound Kinesins in Cultured Hippocampal Neurons.- Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons.- Use of Microfluidics Chambers to Image Axonal Transport In Adult Sensory Neurons.- High-resolution Imaging of Mitochondria and Mitochondrial Nucleoids in Differentiated SH-SY5Y Cells.- Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons.- In vivo Live Imaging of Axonal Transport In Developing Zebrafish Axons.- Visualizing the Intracellular Trafficking in Zebrafish Mauthner Cells.- Dissection and Direct Imaging of Axonal Transport in Drosophila Segmental Nerves.- Detailed Imaging of Mitochondrial Transport and Precise Manipulation of Mitochondrial Function with Genetically-Encoded Photosensitizers in Adult Drosophila Neurons.- Mitochondrial DNA Transport in Drosophila neurons.- Live imaging of Axonal Transport in the Adult Drosophila Central Nervous System.- Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport.- High-resolution Live-Imaging of Axonal RNP granules in Drosophila Pupal Brain Explants.- Analyzing the Impact of Gene Mutations on Axonal Transport in Caenorhabditis elegans.- Methods to Quantify and Relate Axonal Transport Defects to Changes In C. Elegans Behavior.- Imaging Intracellular Trafficking in Neurons of C . elegans .- In vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport.- In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport.