This laboratory manual includes the latest tools and techniques involved in genomic research. It starts with an introductory chapter on genomics and the various tools and applications involved. The initial chapters present protocols for basic techniques such as DNA isolation, electrophoresis, PCR, cDNA synthesis etc. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc. Each topic includes a brief description, information on the principles involved, materials & methods, protocol, and expected results, with diagrams and graphs. All protocols are presented in a very lucid and precise way, to make it easy for readers to follow and replicate them.

Provides up-to-date, step-by-step protocols for both basic and advanced genomics Describes relevant immunology and bioinformatics tools needed to validate genomic studies Includes sections on nutrigenomics, gene therapy, gene editing, and protein purification and analysis Facilitates learning through troubleshooting tips and common pitfalls

Autorentext

Dr Aruna Pal is an Assistant Professor at West Bengal University of Animal Fishery Sciences, India. She has served as a Scientist & Convener, Human Genetics Task Force at the Department of Biotechnology, Ministry of Science & Technology, Govt. of India. She has also worked as a Veterinary Officer at the Govt. of West Bengal and headed the Quality Control Laboratory, Frozen Semen Bull Station, PBGSBS (under the Central Project for Cattle Buffalo Breeding Programme, Ministry of Agriculture, Govt. of India). She was awarded a Junior Research Fellowship (Indian Council of Agricultural Research) for pursuing her M.V.Sc, and a Senior Research Fellowship (Indian Veterinary Research Institute) for pursuing her Ph.D. She has published 21 research papers in highly reputed journals, 2 books, 6 book chapters, 66 gene bank accession numbers (NCBI) and 18 research reviews. A pioneer in the documentation of new breeds of sheep, buffalo and duck from the State of West Bengal, India, she is also an active member of nine scientific societies. She has been the Principal Investigator and co-Principal Investigator for three and six research projects, respectively.

Inhalt
Chapter 1: Conventional and Basic genomic techniques.- Chapter 2: Recombinant DNA technology.- Chapter 3: Mitochondrial Genetics.- Chapter 4: Basic principle of Cloning.- Chapter 5: Immunohistochemistry.- Chapter 6: Western blotting.- Chapter 7: Introduction to Bioinformatics.- Chapter 8: Molecular evolutionary Study-Phylogenetic tree.- Chapter 9: Real Time or Quantitative PCR.- Chapter 10: Tissue or Cell Cultures.- Chapter 11: DNA Micro Array.- Chapter 12: Epigenetics and DNA Methylation.- Chapter 13: RNA sequencing.- Chapter 14: Next Generation sequencing.- Chapter 15: Metagenomics.- Chapter 16: Genome wide association studies (GWAS)/ SNP chips.- Chapter 17: Exome sequencing.- Chapter 18: RNA Interference.- Chapter 19: Expressing cloned genes for protein production, purification and analysis-Biopharming.- Chapter 20: Transgenesis and Biopharming.- Chapter 21: Rapid amplification of cDNA ends (RACE).- Chapter 22: Gene Therapy.- Chapter 23: Nutrigenomics.- Chapter 24: Gene Editing.- Chapter 25: Introduction to stem cell biology.- Chapter 26: Transcriptomics.- Chapter 27: Development of Biomarker.- Chapter 28: Single cell genomics.- Chapter 29: Big data Analysis.