In Animal Models for Stem Cell Therapy: Methods and Protocols, expert researchers in the field detail disease models of hepatic, cardiovascular, neurological diseases, connective and contractile tissue. Chapters focus on a wide range of diseases and application of different kinds of stem cells and reprogrammed tissue cells (iPS). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Animal Models for Stem Cell Therapy: Methods and Protocols, covers interest of basic scientists and clinicians to assess the biological as well as the therapeutic potential of stem cell therapy.

Includes cutting-edge methods and protocols Provides step-by-step details essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Stem Cells--Are We Ready for Therapy?.- Aurigon's Point of View on the Safety Assessment of Cell Based Therapies An Experience Based on the Participation in 15 ATMPs Projects.- Pre-Conditioning of the Liver for Efficient Repopulation by Primary Hepatocyte Transplants.- Age-Dependent Hepatocyte Transplantation for Functional Liver Tissue Reconstitution.- Treatment of NASH with Human Mesenchymal Stem Cells in the Immunodeficient Mouse.- The in vivo Evaluation of the Therapeutic Potential of Human Adipose Tissue-Derived Mesenchymal Stem Cells for Acute Liver Disease.- A Mouse Model of Liver Injury to Evaluate Paracrine and Endocrine Effects of Bone Marrow Mesenchymal Stem Cells.- Animal Models to Test hiPS-Derived Hepatocytes in the Context of Inherited Metabolic Liver Diseases.- Support of Hepatic Regeneration by Trophic Factors from Liver-Derived Mesenchymal Stromal/Stem Cells.- Assessment of Functional Competence of Endothelial Cells from Human Pluripotent Stem Cells in Zebrafish Embryos.- Stem Cell Therapy for Necrotizing Enterocolitis: Innovative Techniques and Procedures for Pediatric Translational Research.- Exploring Mesenchymal Stem Cell-Derived Extracellular Vesicles in Acute Kidney Injury.- Angiogenesic Properties of Mesenchymal Stem Cells in a Mouse Model of Limb Ischemia.- Methods to Assess Intestinal Stem Cell Activity in Response to Microbes in Drosophila melanogaster.- Muscle Pouch Implantation: An Ectopic Bone Formation Model .- Bone Defect Repair in Mice by Mesenchymal Stem Cells.- Generation of Osteoporosis in Immune-Compromised Mice for Stem Cell Therapy.- Application of Stem Cells for the Treatment of Joint Disease in Horses.- Adipogenic Fate Commitment of Muscle-Derived Progenitor Cells: Isolation, Culture and Differentiation.- Skeletal Muscle Stem Cells for Muscle Regeneration.- Bone Marrow Stromal Stem Cells Transplantation in Mice with Acute Spinal Cord Injury.- Histological Characterisation and Quantification ofCellular Events Following Neural and Fibroblast(-Like) Stem Cell Grafting in Healthy and Demyelinated CNS Tissue.- Improvement of Neurological Dysfunctions in Aphakia Mice, a Model of Parkinson's Disease, after Transplantation of ES Cell-Derived Dopaminergic Neuronal Precursors.- Methods for Assessing Regenerative Responses of Neural Tissue.- Mesenchymal Stem Cell-Based Therapy in a Mouse Model of Experimental Autoimmune Encephalomyelitis (EAE).- Analysis of the Neuroregenerative Activities of Mesenchymal Stem Cells in Functional Recovery after Rat Spinal Cord Injury.- Therapeutic Application of Mesenchymal Stromal Cells in Murine Models of Inflammatory Bowel Disease.- Mesenchymal Stem Cells Attenuate Rat Graft-versus-Host Disease.- Assessment of Anti-Donor T Cell Proliferation and Cytotoxic T Lymphocyte-Mediated.- Modulation of Autoimmune Diseases by iPS Cells.- A Chimeric Mouse Model to Study Immunopathogenesis of HCV Infection.