This detailed new edition compiles fully updated practical methodologies and approaches for diagnosticians and researchers working toward a better understanding of animal coronaviruses. The book explores vital techniques in virus isolation, diagnostic approaches via the detection of viral nucleic acids or proteins, serology, disease-targeted animal species models, and next-generation sequencing and bioinformatics. Written for the invaluable Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.
Thorough and hands-on, Animal Coronaviruses, Second Edition will help readers design and carry out their increasingly important projects in clinical research, diagnostics, disease surveillance, and epidemiological study involving this family of viruses.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts

Inhalt

Animal Coronaviruses.- Feline Coronavirus: Insights into the Pathogenesis and Diagnosis.- Animal Models for Studying COVID-19 Pathology and Infection.- Isolation of Porcine Epidemic Diarrhea Virus from Clinical Samples.- Isolation and Characterization of Porcine Epidemic Diarrhea Virus Using Suspension Vero Cells.- Isolation and Propagation of Porcine Deltacoronavirus in Cell Culture and Embryonated Chicken Eggs.- Isolation and Propagation of Infectious Bronchitis Virus (Avian Coronavirus) in Chicken Embryonated Eggs.- Real-Time Reverse Transcription Polymerase Chain Reaction for Simultaneous Detection of Bovine Coronavirus and Other Pathogens.- TaqMan Real-Time RT-PCR Detection of Porcine Epidemic Diarrhea Virus.- Real-Time RT-PCR for Detection of Feline Coronavirus.- SARS-CoV-2 Real-Time RT-PCR Assay in Animals.- Real-Time PCR and Antigen-Capture ELISA for the Diagnosis of Porcine Deltacoronavirus Infection.- Identification of a Neutralizing Epitope-Containing Segment in Turkey Coronavirus Spike Protein for Diagnostic Serology.- Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detecting Protective Antibodies to Turkey Coronavirus Based on Recombinant Spike Protein Segment with Neutralizing Epitope.- ELISA, Virus Neutralization, and Immunofluorescence Assays for Detection of Antibodies Against Porcine Deltacoronavirus.- Luminescent Immunoprecipitation System (LIPS) Development for the Specific Detection of PDCoV and SARS-CoV-2 Antibodies.- Construction of a Mouse-Feline Chimeric Neutralizing Antibody Against SARS-CoV-2.- Microneutralization Test of Avian Infectious Bronchitis Virus Using Vero Cells.- Pig Bioassay to Assess Infectivity of Porcine Epidemic Diarrhea Virus Detected in Various Sample Matrices.- Diagnostic Investigation and Confirmation of Emerging Turkey Coronaviral Enteritis.- Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus.- Whole-Genome Sequencing of Avian Infectious Bronchitis Virus by iSeq100 Platform and MinION.- Phylogenetic and Recombination Analysis of Animal Coronaviruses.