This book is a compendium of novel techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes. The volume's five parts reflect the processes required for different stages of production.

The second edition of this book constitutes a comprehensive manual of new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production.

The chapters are written by world-renowned experts and the volume's five parts reflect the processes required for different stages of production. This book is a compendium of techniques for scientists in both industrial and research laboratories that use mammalian cells for biotechnology purposes.

Chapters written by world-renowned experts Details novel techniques for basic cultivation and complex techniques such as disposable bioreactors, hollow fiber cell culture and fixed bed reactors Provides a state-of-the-art manual for setting up mammalian cell lines for production of biopharmaceuticals Divided into sections to mimic the processes required for different stages of production

Autorentext
Priv.-Doz. Dr.-Ing. Ralf Pörtner, geb. 1958 in Melle. 1978-1984 Studium der Chemietechnik in Dortmund. 1989 Promotion Uni Dortmund, Mechanische Verfahrenstechnik. 1989-1990 Post-Doc Uni Tsukuba, Japan. Seit 1990 Oberingenieur und Leiter Arbeitsgruppe Zellkulturtechnik und Tissue Engineering, TU Hamburg-Harburg, Institut für Bioprozess- und Biosystemtechnik. 2002-2007 Lehrbeauftragter HAW Hamburg. Seit 2008 Lehrbeauftragter an der FH Gießen-Friedberg. 1997 Habilitation und Ernennung zum Privatdozenten. Mitkoordinator des Forschungsschwerpunktes Regeneration, Implantate und Medizintechnik der TU Hamburg-Harburg und Sprecher der Forschungsgruppe Tissue Engineering. Studiengangkoordinator Bioverfahrenstechnik. Etwa 60 referierte Arbeiten sowie über 120 Tagungsbeiträge zu den Themen Zellkulturtechnik, Steuerung von Bioreaktoren, Tissue Engineering.

Inhalt
Basic Techniques for Establishing Production Cell Lines.- Transduction Technologies.- Cells for Gene Therapy and Vector Production.- Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments.- DNA Fingerprinting and Characterization of Animal Cell Lines.- Basic Cultivation Techniques.- Microcarrier Cell Culture Technology.- Cell Encapsulation.- Tools for High-Throughput Medium and Process Optimization.- Cell Characterization and Analysis.- Cell Counting and Viability Measurements.- Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry.- Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors.- Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture.- Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation.- Measurement of Apoptosis in Cell Culture.- Metabolic Flux Estimation in Mammalian Cell Cultures.- Special Cultivation Techniques.- Disposable Bioreactors for Inoculum Production and Protein Expression.- Hollow Fiber Cell Culture.- Cultivation of Mammalian Cells in Fixed-Bed Reactors.- Configuration of Bioreactors.- Downstream Techniques.- Membrane Filtration in Animal Cell Culture.- Chromatographic Techniques in the Downstream Processing of (Recombinant) Proteins.- Special Applications.- Vaccine Production.- Retrovirus Production and Characterization.- Insect Cell Cultivation and Generation of Recombinant Baculovirus Particles for Recombinant Protein Production.