Angiogenesis Protocols

This second edition remains true to its original vision of providing a single source for angiogenesis researchers, collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. However, a number of new chapters are included.

As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity. Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow. Written in the Methods in Molecular Biology™ series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls.

Extensive and cutting-edge, Angiogenesis Protocols, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.

Key in vitro and in vivo techniques covering angio- and lymphangiogenesis Accessible to all levels of expertise User-friendly format/layout Helpful notes based on real experience Well-illustrated up-to-date methods from key leaders in field

Inhalt
Review Articles.- Angiogenic Signalling Pathways.- Angiogenic Signalling Pathways.- Microscopic Assessment.- Assessing Tumor Angiogenesis in Histological Samples.- Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis.- Cell Isolation Techniques.- Vascular Endothelial Cells from Human Micro- and Macro-vessels: Isolation, Characterisation and Culture.- Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established Lines.- Isolation, Culture and Characterisation of Vascular Smooth Muscle Cells.- Circulating Endothelial Progenitor Cells (CEPC).- In vitro Techniques.- In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tubular Differentiation and Proteolysis.- Tube Formation: an In Vitro Matrigel Angiogenesis Assay.- Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model.- Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature.- Genetic Manipulation of Corneal Endothelial Cells: Transfection and Viral Transduction.- Creation of Human Skin Equivalents for the In vitro Study of Angiogenesis in Wound Healing.- In vivo Techniques.- Measurement of Angiogenic Phenotype by Use of a Two Dimensional Mesenteric Angiogenesis Assay.- Quantitative Estimation of Tissue Blood Flow Rate.- In Vivo Matrigel Migration and Angiogenesis Assay.- The Sponge Implant Model of Angiogenesis.- The Dorsal Skinfold Chamber: Studying Angiogenesis by Intravital Microscopy.- The Corneal Pocket Assay.- Use of the Hollow Fibre Assay for Studies on Tumor Neovasculature.- The Cranial Bone Window Model: Studying Angiogenesis of Primary and Secondary Bone Tumors by Intravital Microscopy.