Angiogenesis Protocols

In addition to updating important core techniques this third edition presents new chapters on assessing leukocyte involvement in angiogenesis, lymphatic cell and pericyte isolation techniques, spheroid and arterial ring based in vitro assays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols , Third Edition also guides readers through new protocols on chorioallantoic membrane models, corneal pocket assays to assess angio- and lymphangiogenesis, models of muscle angiogenesis, and use of zebrafish embryos to study vascular angiogenesis and senescence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Angiogenesis Protocols , Third Edition provides not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Assessing Tumor Angiogenesis in Histological Samples.- Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis.- Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis.- Isolation and Culture of Human Endothelial Cells from Micro- And Macro-Vessels.- Isolation, Identification and Culture of Human Lymphatic Endothelial Cells.- Isolation, Culture and Characterisation Of Vascular Smooth Muscle Cells.- Isolation and Transfection of Primary Culture Bovine Retinal Pericytes.- In Vitro Assays for Endothelial Cell Functions Required for Angiogenesis: Proliferation, Motility, Tubular Differentiation And Matrix Proteolysis.- Tube Forming Assays.- In Vitro Co-Culture Assays of Angiogenesis.- Spheroid-Based In Vitro Angiogenesis Model.- Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study Of Angiogenesis.- Human Arterial Ring Angiogenesis Assay.- A Modified Aortic Ring Assay to Assess Angiogenic Potential InVitro.- Quantitative Imaging-Based Examination of Pericyte Control of Endothelial Growth Dynamics and Angiogenesis.- Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature.- Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy.- Quantitative Estimation of Tissue Blood Flow Rate.- Chorioallantoic Membrane Microtumor Model to Study the Mechanisms of Tumor Angiogenesis, Vascular Permeability, and Tumor Cell Intravasation.- The Rabbit Corneal Pocket Assay.- The Corneal Micropocket Assay: A Model of Angiogenesis and Lymphangiogenesis.- Models of Oxygen Induced Retinopathy in Rodents.- The Sponge Implant Model of Angiogenesis.- Measurement of Angiogenesis, Arteriolargenesis and Lymphangiogenesis Phenotypes by Use of Two-Dimensional Mesenteric Angiogenesis Assay.- In Vivo Models of Muscle Angiogenes.- Use of the Hollow Fibre Assay to Evaluate Agents that Target The Tumor Neovasculature.- Angiogenesis and Senescence in Zebrafish Embryos.