This book provides a timely overview of analytical tools and methodological approaches for studying membrane lipids. It outlines the ground-breaking advances that have been made over the last two decades in high-throughput lipidomics, and in studying lipid-protein interactions, signalling pathways and the regulation of lipid metabolism.

This user-friendly laboratory handbook is an ideal companion for membrane biologists, researchers, students, and clinicians alike. It is also well suited for teaching biochemistry, microbiology and biotechnology courses, making it a must-have for everyone whose work involves lipid research.

Highlights key methods for analyzing the structure & function of membrane lipids Outlines recent advances in analytical tools for lipids analysis With a special focus on lipidomics Well illustrated and easy-to-follow format and style

Autorentext
Prof. Rajendra Prasad, Dean, Faculty of Science, Engineering and Technology and Director of the Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, is a former Professor and Dean of the School of Life Sciences at Jawaharlal Nehru University (JNU), New Delhi, where he served for nearly 40 years in various capacities, which included Chairman of Molecular Medicine and Rector (Pro-Vice-Chancellor) of the University. Holding a Ph.D. from the Central Drug Research Institute, Lucknow, Prof. Prasad has also served as a visiting professors at several international universities and institutes in Belgium, Spain, USA; Germany etc. Prof. Prasad is a recipient of the Royal Society Commonwealth Bursary, Alexander von Humboldt Foundation Fellowship, and DFG-Mercator Professorship. He has also served as a European Commission Focal Point Coordinator, Research Ambassador for Germany, and Vice President of the Indian National Science Academy, New Delhi (2013-16). He is currently President of Society of Biological Sciences (India) He is a leading molecular mycologist and has conducted trend-setting research in the area of antifungal clinical drug resistance.

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Dr. Ashutosh Singh is currently an Assistant Professor at the Department of Biochemistry, University of Lucknow, after completing a postdoctoral fellowship at the Department of Molecular Genetics and Microbiology, Stony Brook University, New York, USA. He holds a Ph.D. from the School of Life Sciences at Jawaharlal Nehru University (JNU), New Delhi and was a  ASM sponsored visiting research fellow at the Kansas Lipidomics Research Center, Division of Biology, KSU, Manhattan, USA. Dr. Singh has received many national and international fellowships and awards, including the ASM Young Ambassador to India and is an active member of several scientific societies in his field. His current research focus is on lipid metabolism and fungal pathogenesis. 

Inhalt

Background of Membrane Lipids.- Lipid Regulation in Pathogenic Fungi.- Sphingolipids: Functional and Biological Aspects in Mammals, Plants and Fungi.- Insights into Yeast Phospholipid Tra(ff)icking.- What Can MS, NMR and TLC Tell Us About the Composition of Lipid Membranes?.- Analysis of Sterols by Gas Chromatography - Mass Spectrometry.- Quantitation of Sphingolipids in Mammalian Cell Lines by Liquid Chromatography Mass-Spectrometry.- Exploring Membrane Lipid and Protein Diffusion by FRAP.- Cyclodextrins for Probing Plasma Membrane Lipids.- New Family of Fluorescent Probes for Characterizing Depth-Dependent Static and Dynamic Properties of Lipid/Water Interfaces.- Two-Dimensional Infrared Spectroscopy of Nitrile Labels as a Tool to Probe Dynamics and Interactions in Lipid Membranes.- Estimation and Imaging Techniques to Study Lipids in Mammalian Samples.