This book offers an in-depth exploration of chitosan nanoparticles as an innovative therapeutic strategy for brucellosis, a notable zoonotic disease impacting both livestock and humans globally. The integration of nanotechnology and veterinary medicine explores the synthesis, characterization, and biological applications of chitosan-based nanocarriers, highlighting their potential to transform antimicrobial treatments. The book's first chapter commences with comprehensive introduction to chitosan, detailing its chemistry, solubility, viscosity, and biological properties, which encompass antibacterial, antioxidant, and anti-inflammatory activities. The book systematically outlines advanced synthesis protocols for chitosan nanoparticles, encompassing various techniques including ionotropic gelation, spray drying, nanoprecipitation, and covalent cross-linking. This analysis rigorously evaluates the methodologies to emphasize their effectiveness, scalability, and contribution to nanoparticle stability and bioactivity. The techniques for characterization are essential for comprehending nanoparticle behavior. The third chapter offers an in-depth exploration of size analysis, dynamic light scattering, atomic force microscopy, surface charge determination, and morphological studies utilizing scanning and transmission electron microscopy. These tools are crucial for enhancing nanoparticle design for targeted drug delivery. The fourth chapter focuses on bovine brucellosis, detailing its historical background, etiology, transmission, pathogenesis, and zoonotic potential of Brucella infections. The discussion encompasses contemporary diagnostic methods, therapeutic barriers, and management strategies, highlighting the necessity for novel treatment options. The primary emphasis of the book is on the function of chitosan nanoparticles in the treatment of brucellosis. The fifth chapter investigates nano-drug delivery systems, in vivo experimentation, and drug-loaded chitosan nanoparticles aimed at targeting Brucella infections. The proposed mechanism of action illustrates the methods by which these nanoparticles improve drug bioavailability, facilitate controlled release, and address antimicrobial resistance, and finally, the book concludes with an analysis of current challenges and future research directions, offering an outline for the integration of nanomedicine into veterinary and biomedical sciences. This book serves as a crucial resource for veterinary researchers, microbiologists, pharmaceutical scientists, and experts in nanomedicine who are pursuing advanced strategies to address brucellosis through innovative nanoparticle-based therapies.

Provides a cutting-edge review of chitosan nanoparticles for targeted brucellosis treatment Covers advanced synthesis, characterization, and drug delivery mechanisms of chitosan-based nanocarriers Bridges nanotechnology and veterinary medicine to combat antimicrobial resistance in Brucella infections

Autorentext

Dr. Ali Haider obtained his master's degree (M.Phil., clinical medicine) from University of Veterinary and Animal Sciences (UVAS), Pakistan, in 2015. He obtained his Ph.D. degree in clinical medicine from the Department of Veterinary Medicine (UVAS), Lahore, in 2021. He served as Dairy Farm Manager at reputed commercial dairies of Punjab (Pakistan). Later on (January 2022to date), Ali joined the Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture Multan (MNS-UAM), as Assistant Professor of clinical medicine. His research interest involves the veterinary clinical medicine, green synthesis and characterization of inorganic semiconductor nanomaterials, polymeric nanostructures, 2D materials for their catalytic, antiparasitic, and antibacterial evaluation against multiple drug-resistant (MDR) pathogens.

Dr. Iram Shahzadi obtained her M.Phil. in pharmaceutical chemistry from Punjab University College of Pharmacy (PUCP), University of the Punjab, Lahore, Pakistan, in 2019 and awarded with University Gold Medal. She completed her Ph.D. from PUCP in 2024. In November 2023, Iram joined the School of Pharmacy, University of Management and Technology (UMT), Lahore, as Assistant Professor of Pharmaceutical Chemistry. Iram was recognized among the top 2% of scientists worldwide in 2024 by Stanford University and Elsevier. Her research interests encompass green synthesis, polymeric nanocomposite synthesis and characterization for water treatment, antioxidant, anti-inflammatory activities. She also focuses on drug loading and release mechanisms for anti-cancer drugs, exploring their cytotoxicity potential against various cell lines and computational studies as in silico modeling and density functional theory (DFT) studies.

Sehrish Kiran obtained her B.S. (chemistry) degree from Lahore College for Women University (LCWU), Lahore (Punjab, Pakistan), in 2020. Sehrish completed her master's degree (M.S. in chemistry) from LCWU, Pakistan, in 2022 with research work focused on removal of heavy metals from wastewater using agricultural waste. Her research interest includes synthesis and characterization of polymeric and green nanomaterials with targeted drug delivery and therapeutics, catalytic degradation, and bactericidal evaluations.

Kashaf Shakoor obtained her B.S. degree in zoology from Government College University, Faisalabad (GCUF), Punjab, Pakistan, in 2023. She is now enrolled as M.S. Zoology Student in Muhammad Nawaz Shareef University of Agriculture, Multan (MNS-UAM), Pakistan. Her research interest focuses on synthesis and characterization of polymeric nanostructures with their catalytic and bactericidal evaluations to overcome antibacterial resistance in livestock and fish industry.

Inhalt

1. Chitosan: Introduction.- 2. Synthesis Protocol.- 3. Characterization of Chitosan Nanoparticles.- 4. Bovine Brucellosis.- 5. Chitosan Nanoparticles for Brucellosis Treatment.