Agrobacterium Protocols

Second Edition, Volume II!

A gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Includes supplementary material: sn.pub/extras

Inhalt
Root Plants.- Carrot (Daucus carota L.).- Cassava (Manihot esculenta Crantz).- Potato (Solanum tuberosum L.).- Sweet Potato [Ipomoea batatas (L.) Lam.].- Turf Grasses.- Bermudagrass (Cynodon spp.).- Perennial Ryegrass (Lolium perenne L.).- Switchgrass (Panicum virgatum L.).- Tall Fescue (Festuca arundinacea Schreb.).- Turf Grasses.- Woody Species.- American Elm (Ulmus americana).- Cork Oak Trees (Quercus suber L.).- Eucalyptus.- Pine (Pinus radiata).- Poplar (Populus spp.).- Rubber Tree (Hevea brasiliensis Muell. Arg).- Tropic Plants.- Banana (Musa sp.).- Citrus.- Coffee (Coffea sp.).- Papaya (Carica papaya L.).- Pineapple [Ananas comosus (L.) Merr.].- Sugarcane (Saccharum spp.).- Nuts and Fruits.- American Chestnut [Castanea dentata (Marsh.) Borkh.].- Apple (Malus × domestica).- Blueberry (Vaccinium corymbosum L.).- Grapevine (Vitis vinifera L.).- Strawberry (Fragaria × ananassa).- Walnut (Juglans).- Ornamental Plants.- Carnation (Dianthus caryophylus L.).- Chrysanthemum (Dendranthema × grandiflora).- Orchids (Cymbidium spp., Oncidium, and Phalaenopsis).- Petunia (Petunia hybrida).- Rose (Rosa hybrida L.).- Medicinal Plants.- Ginseng (Panax ginseng).- Hemp (Cannabis sativa L.).- Opium Poppy (Papaver somniferum).- Non-Plants.- Actinomycetes (Streptomyces lividans).- Filamentous Fungi (Magnaporthe grisea and Fusarium oxysporum).- Green Alga (Chlamydomonas reinhardtii).- Mammalian Cells.- Mushroom (Agaricus bisporus).- Yeast (Saccharomyces cerevisiae).