Advances in Bacterial Paracrystalline Surface Layers

This book is a compilation of the research which was presented during the NATO-Advanced Research Workshop (ARW) entitled "Advances in Bacterial Paracrystalline Surface Layers" held in London, Ontario, Canada during September 27 to 30, 1992. The organizing committee consisted of the two Workshop directors, S. F. Kaval and T. J. Beveridge, and H. König, U. B. Sleytr and T. J. Trust; their summary statements about the significance and success of the NATO-ARWare in Chapter 37 of this book. This was the third international workshop on bacterial S-layers and it demonstrated unequivocally how rapidly research is progressing. The Workshop was made possible by financial support from the North Atlantic Treaty Organization (NATO), the Medical Research Council of Canada (MRC), the Natural Seiences and Engineering Research Council of Canada (NSERC), and the Canadian Bacterial Diseases Network (CBDN) which is a Canadian National Centre of Excellence (NCE). We are very grateful for the support from all of these agencies since their financial aid made it possible to bring to London, Canada a truly international group of S-layer experts. We encouraged the attendance and participation of graduate fellows and research associates, and their presentations students, postdoctoral was an intense three constitute the "Poster" section of this book. The NATO-ARW day workshop held at a delightful secluded location (Spencer Hall) so that the delegates had both formal and informal occasions to interact and evolve new ideas.

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1. A Perspective on S-Layer Research.- 2. Crystallographic Image Processing Applications for S-Layers.- 3. Structures of Paracrystalline Protein Layers from the Hyperthermophilic Archaeobacterium Pyrobaculum.- 4. S-Layers Found on Clinical Isolates.- 5. A Common Structural Principle in the Surface Layers of the Archaeobacteria Haloferax, Halobacterium, and Archaeoglobus.- 6. Crystalline Surface-Layers of the Genus Lactobacillus.- 7. Ultrastructural and Chemical Characterization of a Cyanobacterial S-Layer Involved in Fine-Grain Mineral Formation.- 8. Advances in S-Layer Research of Chroococcal Cyanobacteria.- 9. Predation on Bacteria Possessing S-Layers.- 10. Glycoprotein Nature of Select Bacterial S-Layers.- 11. S-Layer Glycoproteins from Moderately and Extremely Halophilic Archaeobacteria.- 12. The Unique Chemical Formats and Biosynthetic Pathways of Methanogenic Surfaces.- 13. Paracrystalline Layers of Methanospirillum hungatei GP1.- 14. Structural and Functional Analysis of the S-Layer Protein from Bacillus stearothermophilus.- 15. Structure-Function Aspects of the Aeromonas salmonicida S-Layer.- 16. Molecular, Structural and Functional Properties of Aeromonas S-Layers.- 17. Biology of Campylobacter fetus S-Layer Proteins.- 18. Definition of Form and Function for the S-Layer of Caulobacter crescentus.- 19. S-Layers as Immobilization and Affinity Matrices.- 20. Molecular Nanotechnology with S-Layers.- 21. Surface Layers from Bacillus alvei as a Carrier for Streptococcus pneumoniae Conjugate Vaccine.- 22. Scale-up of S-Layer Protein Secretion by Bacillus brevis 47.- 23. Investigation of Lattice Surface Layers by Scanning Probe Microscopy.- 24. Stable Liposomes Formed from Archaeal Ether Lipids.- 25. Structural Analysis of the Paracrystalline Layer of Campylobacter fetus subsp. venerealis UA809.- 26. Effect of Triton X-100 on the S-Layer of Methanoculleus marisnigri.- 27. Characterization of the S-Layer Glycoproteins of Two Lactobacilli.- 28. Does the S-Layer of Aeromonas salmonicida Exist in More Than One Functional Organizational State?.- 29. Attachment of the S-Layer of Caulobacter crescentus to the Cell Surface.- 30. Linker Mutagenesis of the Caulobacter crescentus S-Layer Protein.- 31. Can S-Layers of Bacillaceae Control the Release of Their Own Exoproteins?.- 32. S-Layer of Bacillus stearothermophilus PV72.- 33. Role of a C-Terminal Domain in the Structure and Surface Anchoring of the Tetragonal S-Layer of Aeromonas hydrophila.- 34. Identification and Characterization of an Aeromonas salmonicida Gene which Affects A-Protein Expression in Escherichia coli.- 35. Localisation and Cloning of Genes Involved in the Export of the A-Protein of Aeromonas salmonicida.- 36. Growth Medium Considerations for the Scale-up of S-Layer Protein Production by Bacillus brevis 47.- 37. Summary Statements.- Contributors.