This edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes. This book is divided in 8 sections offering a selection of technologies widely used in the characterization of protein and protein-nucleic acid complexes for different purposes and at different scales. From native electrophoresis methods, that are accessible to any reasonably well-equipped laboratory, to the sophisticated setup required for structure determination by cryo-electron microscopy or X-ray crystallography, this book contains a wide variety of clearly explained analytic and preparative approaches, connected with the production techniques developed on the previous volume.

The readers will find an integral connection between this book and the first volume, which ensures a comprehensive and updated discussion of the main topics of the discipline. Taken together, these volumes constitute a cohesive and authoritative source of the research on multicomponent macromolecular complexes. In here, we focus on characterization of protein complexes in the broadest sense, which is not typically covered in other sources. Moreover, all chapters are carefully written by world renowned scientists and active researchers, making this volume ideal, not only as a reference source, but also as a companion book for the daily laboratory work.

This book is aimed for a wide range of scientists, from science students to experienced researchers, working on protein and protein-nucleic acid complexes, who need a thorough understanding of protein production and complex characterization.

Useful companion to the first volume on Protein Complex Production Focuses on the characterization of protein complexes Written by renowned active scientists in the field

Autorentext
Dr. M. Cristina Vega leads a research group at the Chemical and Structural Biology Department, part of the Center for Biological Research Margarita Salas (CIB-CSIC) in Madrid, Spain. Her research blends biochemical, biophysical, and structural biology techniques to explore the mechanisms by which pathogens circumvent the human complement system and other immune defenses and to develop innovative diagnostic and therapeutic approaches.

Dr. Francisco J. Fernández, as the CEO of Abvance Biotech SL, a cutting-edge biopharmaceutical company in Madrid, Spain, brings his extensive expertise in structural biology and recombinant protein production and characterization to the forefront. His work is distinguished by a profound interest in applying synthetic and computational biology tools and techniques for therapeutic antibody development. Dr. Fernández's innovative approach in these fields underpins his leadership in advancing biotechnological applications in medicine.

Inhalt
Chapter 1: Immunoprecipitation methods to isolate messenger ribonucleoprotein complexes (mRNP).- Chapter 2: Purification of in vivo or in vitro-assembled RNA-protein complexes by RNA centric methods.- Chapter 3: Peptide-based mass spectrometry for protein complexes investigation.- Chapter 4: Probing protein complexes composition, stoichiometry, and interactions by peptide-based mass spectrometry.- Chapter 5: Discovery and examination of linear motif mediated protein-protein complexes.- Chapter 6: Protein-protein binding kinetics by biolayer interferometry.- Chapter 7: Studying Macromolecular Interactions of Cellular Machines by the Combined use of Analytical Ultracentrifugation, Light Scattering, and Fluorescence Spectroscopy Methods.- Chapter 8: The complementarity of nuclear magnetic resonance and native mass spectrometry in probing protein-protein interactions.- Chapter 9: X-ray crystallography for protein complexes.- Chapter 10: Characterizationof biological samples using ultra-short and ultra-bright XFEL pulses.- Chapter 11: Small-angle X-ray scattering for macromolecular complexes.- Chapter 12: Sample Preparation for Electron Cryo-Microscopy of Macromolecular Machines.- Chapter 13: Characterization of Complexes and Supramolecular Structures by Electron Microscopy.