3D Sponge-Matrix Histoculture

This volume describes numerous applications of sponge-matrix histoculture to study cancer biology and the treatment of cancer, stem cells, organoids, growth and repair of nerves, lymphoid tissues that produce antibodies, and HIV infection. The chapters in this book cover topics including, in vivo-like growth patterns of multiple types of tumors in Gelfoam® histoculture; development of the histoculture drug response assay (HDRA) for cancer patients; hair-shaft growth in Gelfoam® histoculture of skin as well as isolated hair follicles in Gelfoam® histoculture; the use of Gelfoam® histoculture to discover novel treatment of HIV infection; and imaging DNA repair after UV irradiation damage of cancer cells in Gelfoam® histoculture. The volume explains what is true 3D cell and tissue culture and corrects widespread misconceptions of 3D culture in the literature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cuttingedge and authoritative, 3D Sponge-Matrix Histoculture: Methods and Protocols is a valuable resource that contributes to new cell and tissue culture research, and other diseases based on the true 3D culture.

Includes cutting-edge methods and protocols provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Autorentext
Robert M. Hoffman, Ph.D. Professor of Surgery, University of California, San Diego President, AntiCancer, Inc. 7917 Ostrow Street San Diego, CA 92111, USA

Inhalt
In Memorium: Joseph Leighton, 1921-1999 Father of 3-Dimensional Tissue Culture.- 3D Sponge-Matrix Histoculture: An Overview.- In Vivo-Like Growth Patterns of Multiple Types of Tumors in Gelfoam® Histoculture.- Expression and Targeting of Tumor Markers in Gelfoam® Histoculture: Potential Individualized Assays for Immuno-Oncology.- Development of the Histoculture Drug Response Assay (HDRA).- Diagnosis and Pathological Analysis of Patient Cancers by Detection of Proliferating Cells in Gelfoam® Histoculture.- Clinical Correlation of the Histoculture Drug Response Assay in Gastrointestinal Cancer.- Prospective Clinical Correlation of the Histoculture Drug Response Assay for Ovarian Cancer.- Clinical Correlation of the Histoculture Drug Response Assay for Head and Neck Cancer.- Clinical Usefuleness of the Histoculture Drug Response Assay for Breast Cancer.- Clinical Usefuleness of the Histoculture Drug Response Assay for Prostate Cancer and Benign Prostate Hypertrophy (BPH).- In Vivo-Like Cell Cycle Phase Distribution of Cancer Cells in Gelfoam® Histoculture Observed in Real-Time by FUCCI Imaging.- Methionine Dependency Determination of Human Patient Tumors in Gelfoam® Histoculture.- Hair-Shaft Growth in Gelfoam® Histoculture on Skin and Isolated Hair Follicles.- Histoculture of Hair-Follicle-Associated Pluripotent (HAP) Stem Cells in Gelfoam® Histoculture for Use in Spinal Cord Repair.- Nerve Growth and Interaction in Gelfoam® Histoculture: A Nervous System Organoid.- Histoculture and Infection with HIV of Functional Human Lymphoid Tissue on Gelfoam®.- Imaging DNA Repair after UV Irradiation Damage of Cancer Cells in Gelfoam® Histoculture.- Comparison of Dimensionality of Cancer Cell Culture in Gelfoam® Histoculture and Matrigel.- Imaging the Governing Step of Metastasis in Gelfoam® Histoculture.