The development of effective cardiovascular therapeutics to treat or prevent atherosclerosis and restenosis relies on preclinical research using animal models. In Vascular Disease and Injury: Preclinical Studies, a panel of expert clinical researchers offers a practical guide to the key animal models used in vascular disease research. These experts examine critical issues related to vascular disease and injury in five major areas: acute mechanical injury and vascular repair, arterial thrombosis, chronic atherosclerosis, vascular disease in transplanted vessels, and vascular disease in systemic and pulmonary arterial hypertension. Each practically oriented chapter includes equipment lists; current sources for animals, diet, and reagents; schematic diagrams; and, when pertinent, photomicrographs of sample histology. Key points regarding survival strategy, choice of anesthetic and analgesia, and veterinary issues are also provided, as are the basic histopathologic methods for tissue harvesting, fixation, and electron microscopy preparation.
Up-to-date and highly practical, Vascular Disease and Injury: Preclinical Studies provides a comprehensive review that not only illuminates the current status of cardiovascular research, but also offers vascular biologists and cardiologists a detailed how-to guide to the development of the many powerful new therapeutics now emerging.

Klappentext

A panel of expert clinical researchers offer a practical guide to the key animal models used in vascular disease. These experts examine critical issues related to vascular disease and injury in five major areas: acute mechanical injury and vascular repair, arterial thrombosis, chronic atherosclerosis, vascular disease in transplanted vessels, and vascular disease in systemic and pulmonary arterial hypertension. Up-to-date and highly practical, Vascular Disease and Injury: Preclinical Studies provides a comprehensive review that not only illuminates the current status of cardiovascular research, but also offers vascular biologists and cardiologists a detailed how-to guide to the development of the many powerful new therapeutics now emerging.

Inhalt

Part I. Acute Mechanical Injury and Vascular Repair. Mechanical Injury in Normal and Atherosclerotic Rabbit Iliac Arteries, Frederick G.P. Welt, Elazer R. Edelman, and Campbell Rogers. The Porcine Model of Coronary Restenosis, Robert S. Schwartz, Birgit Kantor, and David R. Holmes Jr. Atherosclerotic Porcine Coronary Stent Model: Technical Methods for Preclinical Studies, Andrew J. Carter. Nonhuman Primate Models of Arterial Injury and Repair, Mark Nedelman, Christopher Horvarth, and Campbell Rogers. Experimental Models of Arteriovenous Grafting, Afshin Ehsan, Michael J. Mann, and Victor J. Dzau. Wire Denudation and Ligation Models of the Mouse Carotid Artery, Volkhard Lindner. Endothelial Denudation and Arterial Dilation in the Mouse, Zhiping Chen, Campbell Rogers, and Daniel I. Simon. Vascular Photochemical Injury in the Mouse, Daniel T. Eitzman and Randal J. Westrick. Mouse Model of Transluminal Femoral Artery Injury, Ernane D. Reis, Susan S. Smyth, and Barry S. Coller. Part II. Models of Arterial Thrombosis. A Murine Model of Arterial Injury and Thrombosis, William P. Fay, Andrew C. Parker, and Yanhong Zhu. Folts Cyclic Flow Animal Model for Studying In Vivo Platelet Activity and Platelet Interactions with Damaged Arterial Walls, John D. Folts. Part III. Chronic Atherosclerotic Models. Mouse Models of Atherosclerosis, Wulf Palinski, Claudio Napoli, and Peter D. Reaven. Rabbit Models of Atherosclerosis, Masanori Aikawa, Yoshihiro Fukumoto, Elena Rabkin, and Peter Libby. Atherosclerosis in Nonhuman Primates, David Kritchevsky and Robert J. Nicolosi. Part IV. Vascular Disease in Transplanted Vessels. Murine Carotid Loop Model of Transplant Vascular Disease, Victor Chengwei Shi and Jennifer L. Hoover. Murine Heterotopic Model of Transplant Arteriopathy, Richard N. Mitchell. Hyperacute Vascular Rejection: Lessons from Pig-to-Primate Xenotransplantation, Raymond H. Chen and David H. Adams. Part V. Vascular Disease in Models of Arterial Hypertension. Methods for Examining Cerebrovascular Disease in Experimental Models of Hypertension, Gary L. Baumbach. Monocrotaline-Induced Pulmonary Hypertension in Rats, Marlene Rabinovitch. Chronic Pulmonary Hypertension in the Hypoxic Rat and in the Sheep Following Continuous Air Embolization, Barbara Meyrick and Elena Tchekneva. Part VI. Animal Care and Tissue Processing. Veterinary Issues and Anesthesia Options, Robert P. Marini. Histological and Immunohistochemical Methods for Vascular Animal Model Studies, Philip Seifert, Campbell Rogers, and Elazer R. Edelman. Index.