Urine Proteomics in Kidney Disease Biomarker Discovery

This book systematically summarizes the ideas and technologies used in urine proteome analysis. It argues that change is the core of biomarker definition since the body uses its homeostatic mechanisms to correct changes in the blood. This means that urine is probably a better source of biomarkers than blood. A roadmap to the urinary biomarker era is proposed, and researchers are reminded of the potential opportunities and risks in their study design. Kidney diseases are emphasized as they produce the most significant changes in urine. This book tries to show researchers and graduate students, who are in or entering the field, "all things considered" rather than "the current affair".

Autorentext
Youhe Gao is a professor from Department of Physiology and Pathophysiology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. He is the member of the standing committee of the proteomics branch of the Chinese Society of Biochemistry and Molecular Biology. He was awarded the National Science Fund for Distinguished Young Scientists in 2007. His research is focused on human urine proteomics and its application, cell secretome and biological predication of protein-protein interaction.

Inhalt

Urine is a better biomarker source than blood especially for kidney diseases.- Urine reflection of changes in blood.- Urimem facilitates kidney disease biomarker research.- Human Urine proteome: a powerful source for clinical research.- Exosomes in urine biomarker discovery.- Urinary proteins with post-translational modifications.- Applications of peptide retention time in proteomic data analysis.- Urine Sample Preparation in 96-Well Filter Plates to characterize inflammatory and infectious diseases of the Urinary Tract.- Variations of human urinary proteome.- Evolution of the urinary proteome during human renal development and maturation.- Hormone-dependent changes in female urinary proteome.- Effects of exercise on the urinary proteome.- Effects of Diuretics on Urinary Proteins.- Applications of urinary proteomics in renal disease research using animal models.- The application of urinary proteomics for the detection of biomarkers of kidney diseases.- Dynamic changes of urinary proteins in focal segmental glomerulosclerosis model.- Using isolated rat kidney to discover kidney origin biomarkers in urine.- Comparing plasma and urinary proteomes to understand kidney function.- Urinary Protein Biomarker Database: A Useful Tool for Biomarker Discovery