Proceedings of the NATO Advanced Study Institute on Tyrosine Phosphorylation/Dephosphorylation and Downstream Signalling, held at Acquafredda di Maratea, Italy, September 1-12, 1992

Inhalt

I. Protein Tyrosine Kinases and Protein Tyrosine Phosphate Phosphatases.- Protein tyrosine phosphorylation-dephosphorylation and cell signaling.- Regulation and functions of src-family kinases: An update.- Mitogen-activated signalling pathways.- Studies of the very early responses of a receptor tyrosine kinase to growth factor binding and their application to the purification and identification of proteins that are tyrosine phosphorylated in the growth factor response.- The role of P21ras in cell signalling.- The HGF/SF receptor kinase: Structure and function.- RET/ptc and TRK oncogenes in papillary thyroid carcinoma.- Structure-function analysis of hepatocyte growth factor and its tyrosine-ldnase receptor c-Met.- Oncogenic activation of the Met/HGF receptor tyrosine kinase is promoted by leucine zipper mediated dimerization.- Specificity of the truncated form of human T-cell protein tyrosine phosphatase toward phosphorylated synthetic peptides.- Regulation of tyrosine protein phosphatases by binding to phospholipids and covalent modification.- Localization-dependent regulation of lymphocyte tyrosine phosphatases.- Protein tyrosine phosphatases in the nematode ascaris suum.- Non-radioactive determination of phosphotyrosine phosphatase (PTPase) activity.- New protein tyrosine phosphatases from vascular smooth muscle cells: Transcript levels in tissues and in cultured cells.- Recombinant CSK expressed in E. Coli is phosphorylated on tyrosine residue(s) and undergoes in vitro phosphorylation.- Polysine activates a splenic tyrosine protein kinase encoded by the fgr protooncogene.- Protein phosphorylation in the signal transduction of the neuropeptide bombesin in swiss 3T3 cells.- Growth hormone (GH) increases lipoprotein lipase mRNA in cells transfected with the rat GH receptor cDNA.- II. The Phosphatidylinositol Signalling System.- Role of phosphatidylinositol 3-kinase in growth factor and oncogene signaling.- Isolation and characterization of phosphatidylinositol 4-kinase isoenzymes from bovine brain.- Determination of phosphoinositides and inositol phosphates. Principles and rules.- Phosphoinositides in rabbit skeletal muscle membranes.- Renaturation of phosphatidylinositol 4- and phosphatidylinositol 4-phosphate 5?-kinases following polyacrylamide gelelectrophoresis in presence of SDS. Studies on their substrate binding requirements using synthetic substrate analogues.- III. Serine/Threonine Protein Kinases.- The catalytic subunit of cAMP-dependent protein kinase.- Casein kinase I and the classification of protein kinases: What's in a name?.- Downregulation of protein kinase C is associated with phenotypic changes and enhanced proliferation of vascular smooth muscle cells.- Insulin-stimulated seryl/threonyl protein kinases in diabetic rats and the effects of vanadium treatment.- Protein phosphorylation in prokaryotes - evidence for pkc-like activities in Escherichia Coli.- Phosphorylation of creatine kinase in myogenic cells: Effects of okadaic acid and other agents affecting cellular protein phosphorylation.- Non-radioactive determination of phosphoamino acids by capillary electrophoresis.- Characterization of phosphoproteins by 31P-NMR spectroscopy.- CK-2, a multifunctional protein kinase and its role during proliferation.- IV. Protein Serine/Threonine Phosphate Phosphatases.- Molecular complexity of protein phosphatase 2A holoenzymes and delineation of their function.- Dephosphorylation in vitro inhibits ligand and DNA binding activities of human estrogen receptor.- Characterization of novel nuclear inhibitors of protein phosphatase-1.- V. S6-Phosphorylation.- The mitogen-activated p70s6k / p85s6k.- Expression of the p70s6k / p85s6k isoforms from a single gene and activation following mitogenic stimulation.- Control of S6 phosphorylation in normal and v-src transformed cells.- The phosphorylated ribosomal protein in tetrahymena is homologous with mammalian ribosomal protein S4.- Nucleocytoplasmic transport of ribosomal protein S6.- VI. Cell-Cycle-Control.- Regulating the G2 checkpoint in the cell cycle.- CD2-dependence on the CD3-zeta-chain in T cell signalling - shown by inhibitory and modifying effects of cholera toxin.- cdc25 protein phosphatase in mammalian fibroblasts: cell cycle expression and intracellular localization.- VII. Control of Intracellular Ca2+.- The role of small molecular weight GTP-binding proteins in stimulus secretion coupling of pancreatic acinar cells.- Characterization of calcium transport ATPase isozymes in different blood cells.