Symbiotic Fungi - Principles and Practice presents current protocols for the study of symbiotic fungi and their interactions with plant roots, such as techniques for analyzing nutrient transfer, ecological restoration, microbial communication, and mycorrhizal bioassays, AM inoculum procedures and mushroom technology.

The protocols offer practical solutions for researchers and students involved in the study of symbiotic microorganisms. The volume will be of great use for basic research, biotechnological applications, and the development of commercial products.

Inhalt
Symbiosis: The Art of Living.- Analysis of Rhizosphere Fungal Communities Using rRNA and rDNA.- Use of Mycorrhiza Bioassays in Ecological Studies.- In Vivo Model Systems for Visualisation, Quantification and Experimental Studies of Intact Arbuscular Mycorrhizal Networks.- Measurement of Net Ion Fluxes Using Ion-Selective Microelectrodes at the Surface of Ectomycorrhizal Roots.- Assessment of Phosphatase Activity Associated with Mycorrhizal Fungi by Epi-Fluorescent Microscopy.- In Vitro Compartmented Systems to Study Transport in Arbuscular Mycorrhizal Symbiosis.- Use of the Autofluorescence Properties of AM Fungi for AM Assessment and Handling.- Role of Root Exudates and Rhizosphere Microflora in the Arbuscular Mycorrhizal Fungi-Mediated Biocontrol of Phytophthora nicotianae in Tomato.- Assessing the Mycorrhizal Diversity of Soils and Identification of Fungus Fruiting Bodies and Axenic Cultures.- Isolation of Metabolically Active Arbuscules and Intraradical Hyphae from Mycorrhizal Roots.- Interaction with Soil Microorganisms.- Isolation, Cultivation and In Planta Visualization of Bacterial Endophytes in Hanging Roots of Banyan Tree (Ficus bengalensis).- Micro-PIXE Analysis for Localization and Quantification of Elements in Roots of Mycorrhizal Metal-Tolerant Plants.- Functional Genomic of Arbuscular Mycorrhizal Symbiosis: Why and How Using Proteomics.- Using Stable Carbon Isotope Labelling in Signature Fatty Acids to Track Carbon Allocation in Arbuscular Mycorrhiza.- 15N Enrichment Methods to Quantify Two-Way Nitrogen Transfer Between Plants Linked by Mychorrhizal Networks.- Analyses of Ecophysiological Traits of Tropical Rain Forest Seedlings under Arbuscular Mycorrhization: Implications in Ecological Restoration.- Techniques for Arbuscular Mycorrhiza Inoculum Reduction.- Best Production Practice of Arbuscular Mycorrhizal Inoculum.- The Use of AMF and PGPR Inoculants Singly and Combined to Promote Microplant Establishment, Growth and Health.- Co-Culture of Linum album Cells and Piriformospora indica for Improved Production of Phytopharmaceuticals.- Fungal Elicitors for Enhanced Production of Secondary Metabolites in Plant Cell Suspension Cultures.- Auxin Production by Symbiotic Fungi: Bioassay and HPLC-MS Analysis.- Siderophores of Mycorrhizal Fungi: Detection, Isolation and Identification.- Biology and Molecular Approaches in Genetic Improvement of Cultivated Button Mushroom (Agaricus Bisporus).