Willkommen, schön sind Sie da!
Logo Ex Libris

Ectoine production by halotolerant microorganisms – Process optimization and characterization of cellular state

  • E-Book (pdf)
  • 148 Seiten
(0) Erste Bewertung abgeben
Bewertungen
(0)
(0)
(0)
(0)
(0)
Alle Bewertungen ansehen
Halophile microorganisms are important ectoine producers and applied in industrial applications. The growth and osmoregulation are... Weiterlesen
E-Books ganz einfach mit der kostenlosen Ex Libris-Reader-App lesen. Hier erhalten Sie Ihren Download-Link.
CHF 19.90
Download steht sofort bereit
Informationen zu E-Books
E-Books eignen sich auch für mobile Geräte (sehen Sie dazu die Anleitungen).
E-Books von Ex Libris sind mit Adobe DRM kopiergeschützt: Erfahren Sie mehr.
Weitere Informationen finden Sie hier.
Bestellung & Lieferung in eine Filiale möglich

Beschreibung

Halophile microorganisms are important ectoine producers and applied in industrial applications. The growth and osmoregulation are distinguished based on the diversity of environmental distribution and thus require holistic investigations of cultivation condition and osmotic response. Alkalibacillus haloalkaliphilus was used to extensively study the production and secretion of the compatible solute ectoine and allowing biological interpretations on bacterial stress response by investigation of the changes within cellular state and membrane characteristics during the stress-related bioprocess. Following the holistic approach, first nutrient requirements of the organism were screened by microarrays, providing an enhanced biological insight in the catabolism and reveals bottlenecks in metabolism, which resulted in a selection of potential industrial raw materials. The most appropriate substrate source was then applied for the optimization process using DoE and RSM. Since microbial growth and ectoine production are functions of the process key parameters salinity, pH-value and temperature, the optimization resulted in three significant process strategies. For each strategy, single-cell analysis was performed using a newly developed staining method for monitoring the change in membrane potential of halophilious microorganisms. Subsequently, the loaded cells were forced to release the accumulated ectoine by combined thermal and hypo- and hyper-osmotic shocks. As the bacterial stress response, combined with physiochemical responses to the altered environment, influence the cell membrane properties, the changes in membrane fluidity and integrity induced by environmental stress were investigated by fluorescent approach, using steady-state anisotropy measurements and singlecell analysis, which leads to optimized secretion conditions. The established single-cell analysis (flow cytometry) and steady-state anisotropy measurement revealed changes of the physiological state during ectoine production and secretion and thus provides a deeper insight in the bacterial stress response.

Produktinformationen

Titel: Ectoine production by halotolerant microorganisms – Process optimization and characterization of cellular state
Autor:
EAN: 9783736945562
ISBN: 978-3-7369-4556-2
Digitaler Kopierschutz: frei
Format: E-Book (pdf)
Herausgeber: Cuvillier Verlag
Genre: Technik
Anzahl Seiten: 148
Veröffentlichung: 11.12.2013
Jahr: 2013
Untertitel: Englisch
Dateigrösse: 4.3 MB