This book constitutes the refereed proceedings of the 16th International Conference on Subject-Oriented Business Process Management, S-BPM ONE 2025, held in Weiden, Germany, during July 2-3, 2025. This year's motto was **Process Customer Satisfaction and Process Excellence.

The 12 full papers and 4 short papers included in this book were carefully reviewed and selected from 29 submissions.They were organized in following topical sections: Subject-Oriented Modeling, Philosophy, and Technology and Human or Artificial Intelligence in BPM.