In the ten years since the publication of the first edition of this book, structural virology has undergone many important conceptual and technical advances. A technical revolution has allowed the determination of the molecular structures of many viruses at very high resolution by cryogenic electron microscopy. The dynamic nature of viral particles is being revealed and analysed by an increasing number of biophysical techniques, such as NMR spectroscopy and mass spectrometry as well as by all-atom molecular dynamics simulations. New advances in correlative microscopy are being applied to probe the structure and dynamics of viruses inside infected cells. The fight against the COVID-19 pandemia has also contributed to stimulate basic and applied research studies on the relationships between the structure and function of viruses.

Physical virology has come of age and offers approaches based on fundamental physics to study different aspects of the architecture, self-assembly, and material properties of viral particles. Atomic force microscopy and optical tweezers are probing the mechanical behavior of viral particles and unraveling their biological implications. High-speed atomic force microscopy is beginning to provide new detailed insights into virus assembly and dynamics. Powerful theoretical approaches and molecular dynamics simulations search for the physical underpinnings underlying viruses' structure and material properties. Structural virology and physical virology are merging with biochemical and biological approaches to provide a renewed and richer view of the relationships between the molecular structure, physical properties, and biological function of viruses. This interdisciplinary approach is also leading to important advances in the fight against viral diseases and to applications of virus-derived particles and virus components in biomedicine, biotechnology and nanotechnology.

Structure and Physics of Viruses, 2nd edition is an interdisciplinary textbook that takes an integrated approach to the rapidly merging and expanding fields of structural and physical virology. This new edition has been updated to include many important technical developments and conceptual advances in these research areas over the past decade. The content of the book has been kept basic enough to be useful to students and advanced enough to be useful to senior scientists. This book is aimed primarily at master's students, Ph.D. students and postdoctoral researchers with undergraduate degrees in biology, chemistry, physics or related sciences who are interested in viruses or are just starting to work with viruses. It also aims to provide an up-to-date overview of many important concepts, techniques, studies, and applications in structural and physical virology for established researchers working with viruses, regardless of their physical, chemical, or biological background and field of specialization.

Autorentext

Mauricio G. Mateu is a Full Professor of Biochemistry and Molecular Biology at the Universidad Autonoma de Madrid (UAM), and a Group Leader at the Centro de Biología Molecular "Severo Ochoa" (Spanish Research Council-UAM). He combined training in biochemistry, molecular virology, and protein engineering in three prestigious laboratories, respectively led by Profs. Severo Ochoa and Esteban Domingo in Madrid, Spain, and Alan R. Fersht in Cambridge, UK, to establish a research line on the structural basis of virus function. His group uses protein engineering and biological, biochemical, and biophysical approaches, including atomic force microscopy, to understand the relationships between atomic structure, conformational stability and dynamics, physical properties, and biological function of viruses. His studies on viruses during the last 35 years have involved multidisciplinary and interdisciplinary collaborations with theoretical and condensed matter physicists, organic chemists, biochemists, structural biologists, biophysicists, and molecular virologists. He is the author of over 150 scientific publications, including over 110 original research articles.